Optimization of a fluorogenic assay to determine caspase 3/7 activity in meat extracts

Usefulness of general-purpose fluorogenic assay kits to determine caspase 3/7 activity of biological extracts is highly compromised in meat-based samples due to their scarce enzyme concentration. In the present work, a straightforward protocol is presented with two main purposes: 1) to enhance sensi...

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Bibliographische Detailangaben

Veröffentlicht in:	Food science and technology international = Ciencia y tecnologia de los alimentos internacional. - 1998. - 28(2022), 2 vom: 01. März, Seite 128-134
1. Verfasser:	Fuente-Garcia, Claudia (VerfasserIn)
Weitere Verfasser:	Sentandreu, Enrique, Aldai, Noelia, Sentandreu, Miguel A
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2022
Zugriff auf das übergeordnete Werk:	Food science and technology international = Ciencia y tecnologia de los alimentos internacional
Schlagworte:	Journal Article Ac-DEVD-AMC peptide substrate Caspase 3/7 activity apoptosis meat extracts method development rapid assays Indicators and Reagents Caspase 3 EC 3.4.22.- Caspases