Characterization of crude extract prepared from Indian curd and its potential as a biopreservative

The adverse effects of chemical preservatives used to prevent food spoilage have led to the search for various biopreservatives. Considering this, a study was undertaken to prepare crude extract (CE) from Indian curd (a fermented dairy product) and characterized it in terms of antioxidant and antimi...

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Publié dans:Food science and technology international = Ciencia y tecnologia de los alimentos internacional. - 1998. - 27(2021), 4 vom: 18. Juni, Seite 313-325
Auteur principal: Das, Annada (Auteur)
Autres auteurs: Chauhan, Geeta, Agrawal, Ravi K, Das, Arun K, Tomar, Serlene, Uddin, Siraj, Satyaprakash, Kaushik, Pateiro, Mirian, Lorenzo, José M
Format: Article en ligne
Langue:English
Publié: 2021
Accès à la collection:Food science and technology international = Ciencia y tecnologia de los alimentos internacional
Sujets:Journal Article Curd extract antimicrobial antioxidant bioactivity biopreservative Anti-Bacterial Agents Bacteriocins Complex Mixtures Food Preservatives
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520 |a The adverse effects of chemical preservatives used to prevent food spoilage have led to the search for various biopreservatives. Considering this, a study was undertaken to prepare crude extract (CE) from Indian curd (a fermented dairy product) and characterized it in terms of antioxidant and antimicrobial activities against some common food-borne bacteria. The CE exhibited well pronounced antimicrobial activity against Bacillus cereus and Salmonella typhimurium. The minimum inhibitory concentration (MIC) of CE was recorded for 2-fold concentrated solution prepared from a 10-fold stock. The CE exhibited a significantly higher (p < 0.05) antioxidant and antimicrobial activities compared to its fractions. The CE was found to be heat stable (up to 100 ℃ for 30 min) and exhibited a significant (p < 0.05) increase in activity at pH 2-7 and in combination with 2% citric acid solution. Trypsin treatment suggested it to be of proteinaceous in nature. The antibacterial activity of CE remained intact at 4 ℃ for seven days, whereas non-significant (p > 0.05) changes in its activity were noted during storage at -20 ℃ for 30 days. The curd sample used for preparation of CE, when tested for bacteriocin production and subsequent antimicrobial activity, did not show inhibition against S. typhimurium. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis analysis of CE and its fractions revealed multi-banding pattern. By virtue of its bioactivities observed, CE can be explored as a promising food biopreservative 
650 4 |a Journal Article 
650 4 |a Curd extract 
650 4 |a antimicrobial 
650 4 |a antioxidant 
650 4 |a bioactivity 
650 4 |a biopreservative 
650 7 |a Anti-Bacterial Agents  |2 NLM 
650 7 |a Bacteriocins  |2 NLM 
650 7 |a Complex Mixtures  |2 NLM 
650 7 |a Food Preservatives  |2 NLM 
700 1 |a Chauhan, Geeta  |e verfasserin  |4 aut 
700 1 |a Agrawal, Ravi K  |e verfasserin  |4 aut 
700 1 |a Das, Arun K  |e verfasserin  |4 aut 
700 1 |a Tomar, Serlene  |e verfasserin  |4 aut 
700 1 |a Uddin, Siraj  |e verfasserin  |4 aut 
700 1 |a Satyaprakash, Kaushik  |e verfasserin  |4 aut 
700 1 |a Pateiro, Mirian  |e verfasserin  |4 aut 
700 1 |a Lorenzo, José M  |e verfasserin  |4 aut 
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773 1 8 |g volume:27  |g year:2021  |g number:4  |g day:18  |g month:06  |g pages:313-325 
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