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231225s2021 xx |||||o 00| ||eng c |
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|a 10.1111/nph.16975
|2 doi
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|a pubmed24n1056.xml
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|a DE-627
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|e rakwb
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|a eng
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|a Rodríguez-López, Carlos E
|e verfasserin
|4 aut
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|a Two bi-functional cytochrome P450 CYP72 enzymes from olive (Olea europaea) catalyze the oxidative C-C bond cleavage in the biosynthesis of secoxy-iridoids - flavor and quality determinants in olive oil
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|c 2021
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 14.05.2021
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|a Date Revised 14.05.2021
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2020 The Authors. New Phytologist © 2020 New Phytologist Foundation.
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|a Olive (Olea europaea) is an important crop in Europe, with high cultural, economic and nutritional significance. Olive oil flavor and quality depend on phenolic secoiridoids, but the biosynthetic pathway of these iridoids remains largely uncharacterized. We discovered two bifunctional cytochrome P450 enzymes, catalyzing the rare oxidative C-C bond cleavage of 7-epi-loganin to produce oleoside methyl ester (OeOMES) and secoxyloganin (OeSXS), both through a ketologanin intermediary. Although these enzymes are homologous to the previously reported Catharanthus roseus secologanin synthase (CrSLS), the substrate and product profiles differ. Biochemical assays provided mechanistic insights into the two-step OeOMES and CrSLS reactions. Model-guided mutations of OeOMES changed the product profile in a predictable manner, revealing insights into the molecular basis for this change in product specificity. Our results suggest that, in contrast to published hypotheses, in planta production of secoxy-iridoids is secologanin-independent. Notably, sequence data of cultivated and wild olives point to a relation between domestication and OeOMES expression. Thus, the discovery of this key biosynthetic gene suggests a link between domestication and secondary metabolism, and could potentially be used as a genetic marker to guide next-generation breeding programs
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Research Support, U.S. Gov't, Non-P.H.S.
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|a Olea europaea
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|a C-C oxidative cleavage
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|a cytochrome P450
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|a iridoid biosynthesis
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|a oleoside methyl ester
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|a oleuropein
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|a olive
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|a phenolic secoiridoids
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|a Iridoids
|2 NLM
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| 650 |
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|a Olive Oil
|2 NLM
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| 650 |
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|a Cytochrome P-450 Enzyme System
|2 NLM
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| 650 |
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|a 9035-51-2
|2 NLM
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| 700 |
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|a Hong, Benke
|e verfasserin
|4 aut
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|a Paetz, Christian
|e verfasserin
|4 aut
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|a Nakamura, Yoko
|e verfasserin
|4 aut
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|a Koudounas, Konstantinos
|e verfasserin
|4 aut
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|a Passeri, Valentina
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|a Baldoni, Luciana
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|a Alagna, Fiammetta
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|a Calderini, Ornella
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|a O'Connor, Sarah E
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t The New phytologist
|d 1979
|g 229(2021), 4 vom: 19. Feb., Seite 2288-2301
|w (DE-627)NLM09818248X
|x 1469-8137
|7 nnns
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| 773 |
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|g volume:229
|g year:2021
|g number:4
|g day:19
|g month:02
|g pages:2288-2301
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|u http://dx.doi.org/10.1111/nph.16975
|3 Volltext
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|d 229
|j 2021
|e 4
|b 19
|c 02
|h 2288-2301
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