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231225s2021 xx |||||o 00| ||eng c |
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|a 10.1177/1082013220929144
|2 doi
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|a eng
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1 |
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|a Sentandreu, Enrique
|e verfasserin
|4 aut
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|a A straightforward gel-free proteomics pipeline assisted by liquid isoelectric focusing (OFFGEL) and mass spectrometry analysis to study bovine meat proteome
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|c 2021
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|a Text
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|a ƒaComputermedien
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|a ƒa Online-Ressource
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|a Date Completed 06.08.2021
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|a Date Revised 06.08.2021
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Bovine sarcoplasmic sub-proteome was studied through a straightforward gel-free pipeline supported by liquid isoelectric focusing (OFFGEL) protein fractionation coupled to liquid chromatography-mass spectrometry (LC-MS) analysis. Full-MS and data-dependent MS/MS analyses were simultaneously performed by a conventional three-dimensional ion-trap addressing targeted quantitative and untargeted qualitative research, respectively. There were unambiguously identified 47 proteins distributed along 12 OFFGEL fractions assayed. Regarding intermediate- and high-abundant peptides, bulky quantitative data processing performed by MZmine 2 freeware yielded a satisfactory linearity and coefficient of variation with r2 in the 0.95-0.99 range and about 25%, respectively. Up to 41 peptides from 20 identified proteins were relatively quantified throughout OFFGEL fractions. This reliable, flexible and affordable gel-free proteomic approach could be readily implemented by industry to improve quality assessment of protein-based food products
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|a Journal Article
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|a Proteomics
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|a chromatography analysis
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| 650 |
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|a food proteins
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|a quality assessment
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| 650 |
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|a Proteome
|2 NLM
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| 700 |
1 |
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|a Fuente-García, Claudia
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Navarro, José L
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Sentandreu, Miguel A
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Food science and technology international = Ciencia y tecnologia de los alimentos internacional
|d 1998
|g 27(2021), 2 vom: 29. März, Seite 112-122
|w (DE-627)NLM098247832
|x 1532-1738
|7 nnas
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| 773 |
1 |
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|g volume:27
|g year:2021
|g number:2
|g day:29
|g month:03
|g pages:112-122
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|u http://dx.doi.org/10.1177/1082013220929144
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