Immunophenotyping of A20 haploinsufficiency by multicolor flow cytometry

Immunophenotyping of A20 haploinsufficiency by multicolor flow cytometry

Copyright © 2020 Elsevier Inc. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Clinical immunology (Orlando, Fla.). - 1999. - 216(2020) vom: 05. Juli, Seite 108441
1. Verfasser: Kadowaki, Tomonori (VerfasserIn)
Weitere Verfasser: Ohnishi, Hidenori, Kawamoto, Norio, Kadowaki, Saori, Hori, Tomohiro, Nishimura, Kenichi, Kobayashi, Chie, Shigemura, Tomonari, Ogata, Shohei, Inoue, Yuzaburo, Hiejima, Eitaro, Izawa, Kazushi, Matsubayashi, Tadashi, Matsumoto, Kazuaki, Imai, Kohsuke, Nishikomori, Ryuta, Ito, Shuichi, Kanegane, Hirokazu, Fukao, Toshiyuki
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2020
Zugriff auf das übergeordnete Werk:Clinical immunology (Orlando, Fla.)
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Autoimmunity Double-negative T cell Follicular helper T cell Haploinsufficiency of A20 Regulatory T cell TNFAIP3
Beschreibung
Zusammenfassung:Copyright © 2020 Elsevier Inc. All rights reserved.
Haploinsufficiency of A20 (HA20) causes inflammatory disease resembling Behçet's disease; many cases have been reported, including some that are complicated with autoimmune diseases. This study aims to clarify the immunophenotype of patients with HA20 by analyzing lymphocyte subsets using multicolor flow cytometry. The patients with HA20 previously diagnosed in a nationwide survey were compared by their cell subpopulations. In total, 27 parameters including regulatory T cells (Tregs), double-negative T cells (DNTs), and follicular helper T cells (TFHs) were analyzed and compared with the reference values in four age groups: 0-1, 2-6, 7-19, and ≥20 years. The Tregs of patients with HA20 tended to increase in tandem with age-matched controls at all ages. In addition, patients ≥20 years had increased DNTs compared with controls, whereas TFHs significantly increased in younger patients. In HA20 patients, the increase in DNTs and TFHs may contribute to the development of autoimmune diseases
Beschreibung:Date Completed 01.02.2021
Date Revised 01.02.2021
published: Print-Electronic
Citation Status MEDLINE
ISSN:1521-7035
DOI:10.1016/j.clim.2020.108441