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231225s2020 xx |||||o 00| ||eng c |
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|a 10.1093/jxb/eraa009
|2 doi
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|a pubmed24n1352.xml
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|a (DE-627)NLM305247123
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|a (NLM)31926021
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
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|a Wu, Wei
|e verfasserin
|4 aut
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|a High CO2/hypoxia-induced softening of persimmon fruit is modulated by DkERF8/16 and DkNAC9 complexes
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|c 2020
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 14.05.2021
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|a Date Revised 28.03.2024
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|a published: Print
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|a Citation Status MEDLINE
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|a © The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology.
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|a Most persimmon (Diospyros kaki) cultivars are astringent and require post-harvest deastringency treatments such as 95% CO2 (high-CO2 treatment) to make them acceptable to consumers. High-CO2 treatment can, however, also induce excessive softening, which can be reduced by adding 1-methylcyclopropene (1-MCP). Previous studies have shown that genes encoding the ETHYLENE RESPONSE FACTORS (ERFs) DkERF8/16/19 can trans-activate xyloglucan endotransglycosylase/hydrolase (DkXTH9), which encodes the cell wall-degrading enzyme associated with persimmon fruit softening. In this study, RNA-seq data between three treatments were compared, namely high-CO2, high-CO2+1-MCP, and controls. A total of 227 differentially expressed genes, including 17 transcription factors, were predicted to be related to persimmon post-deastringency softening. Dual-luciferase assays indicated that DkNAC9 activated the DkEGase1 promoter 2.64-fold. Synergistic effects on transcription of DkEGase1 that involved DkNAC9 and the previously reported DkERF8/16 were identified. Electrophoretic mobility shift assay indicated that DkNAC9 could physically bind to the DkEGase1 promoter. Bimolecular fluorescence complementation and firefly luciferase complementation imaging assays indicated protein-protein interactions between DkNAC9 and DkERF8/16. Based on these findings, we conclude that DkNAC9 is a direct transcriptional activator of DkEGase1 that can co-operate with DkERF8/16 to enhance fruit post-deastringency softening
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a DkEGase1
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|a NAC
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|a NAC-ERF complex
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|a persimmon fruit
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|a post-deastringency softening
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|a transcriptional regulation
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|a transcriptomic analysis
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|a Plant Proteins
|2 NLM
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|a Carbon Dioxide
|2 NLM
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|a 142M471B3J
|2 NLM
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| 700 |
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|a Wang, Miao-Miao
|e verfasserin
|4 aut
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|a Gong, Hui
|e verfasserin
|4 aut
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|a Liu, Xiao-Fen
|e verfasserin
|4 aut
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|a Guo, Da-Long
|e verfasserin
|4 aut
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|a Sun, Ning-Jing
|e verfasserin
|4 aut
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|a Huang, Jing-Wen
|e verfasserin
|4 aut
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|a Zhu, Qing-Gang
|e verfasserin
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|a Chen, Kun-Song
|e verfasserin
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|a Yin, Xue-Ren
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 71(2020), 9 vom: 09. Mai, Seite 2690-2700
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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| 773 |
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|g volume:71
|g year:2020
|g number:9
|g day:09
|g month:05
|g pages:2690-2700
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|u http://dx.doi.org/10.1093/jxb/eraa009
|3 Volltext
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|d 71
|j 2020
|e 9
|b 09
|c 05
|h 2690-2700
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