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231225s2018 xx |||||o 00| ||eng c |
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|a 10.1093/jxb/ery300
|2 doi
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|a pubmed24n0958.xml
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|a (DE-627)NLM287636127
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|a (NLM)30124964
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Ko, Mi Ran
|e verfasserin
|4 aut
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| 245 |
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|a RNAi-mediated suppression of three carotenoid-cleavage dioxygenase genes, OsCCD1, 4a, and 4b, increases carotenoid content in rice
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|c 2018
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 15.10.2019
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|a Date Revised 15.10.2019
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|a published: Print
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|a Citation Status MEDLINE
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|a Carotenoids of staple food crops have a high nutritional value as provitamin A components in the daily diet. To increase the levels of carotenoids, inhibition of carotenoid-cleavage dioxygenases (CCDs), which degrade carotenoids, has been considered as a promising target in crop biotechnology. In this study, suppression of the OsCCD1, OsCCD4a, and OsCCD4b genes using RNAi was verified in transgenic rice plants by quantitative RT-PCR and small RNA detection. Leaf carotenoids were significantly increased overall in OsCCD4a-RNAi lines of the T1 generation, and the highest accumulation of 1.3-fold relative to non-transgenic plants was found in a line of the T2 generation. The effects on seed carotenoids were determined via cross-fertilization between β-carotene-producing transgenic rice and one of two independent homozygous lines of OsCCD1-RNAi, OsCCD4a-RNAi, or OsCCD4b-RNAi. This showed that carotenoids were increased to a maximum of 1.4- and 1.6-fold in OsCCD1-RNAi and OsCCD4a-RNAi, respectively, with a different preference toward α-ring and β-ring carotenoids; levels could not be established in OsCCD4b-RNAi. In addition, the contents of four carotenoids decreased when OsCCD1, OsCCD4a, and OsCCD4b were overexpressed in E. coli strains accumulating phytoene, lycopene, β-carotene, and zeaxanthin. OsCCD1 and OsCCD4a had a similar high carotenoid degrading activity, followed by OsCCD4b without substrate specificity. Overall, our results suggest that suppresing OsCCD4a activity may have potential as a tool for enhancing the carotenoid content of seed endosperms and leaves in rice
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Plant Proteins
|2 NLM
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|a Carotenoids
|2 NLM
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|a 36-88-4
|2 NLM
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|a Dioxygenases
|2 NLM
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| 650 |
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|a EC 1.13.11.-
|2 NLM
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| 650 |
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|a carotenoid cleavage dioxygenase 1
|2 NLM
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| 650 |
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|a EC 1.13.11.-
|2 NLM
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| 700 |
1 |
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|a Song, Mi-Hee
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Kim, Jae Kwang
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Baek, Seung-A
|e verfasserin
|4 aut
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| 700 |
1 |
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|a You, Min Kyoung
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Lim, Sun-Hyung
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Ha, Sun-Hwa
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 69(2018), 21 vom: 12. Okt., Seite 5105-5116
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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| 773 |
1 |
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|g volume:69
|g year:2018
|g number:21
|g day:12
|g month:10
|g pages:5105-5116
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| 856 |
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|u http://dx.doi.org/10.1093/jxb/ery300
|3 Volltext
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