Targeted Editing of Myostatin Gene in Sheep by Transcription Activator-like Effector Nucleases

Myostatin (MSTN) is a secreted growth factor expressed in skeletal muscle and adipose tissue that negatively regulates skeletal muscle mass. Gene knockout of MSTN can result in increasing muscle mass in sheep. The objectives were to investigate whether myostatin gene can be edited in sheep by transc...

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Bibliographische Detailangaben
Veröffentlicht in:	Asian-Australasian journal of animal sciences. - 1998. - 29(2016), 3 vom: 01. März, Seite 413-8
1. Verfasser:	Zhao, Xinxia (VerfasserIn)
Weitere Verfasser:	Ni, Wei, Chen, Chuangfu, Sai, Wujiafu, Qiao, Jun, Sheng, Jingliang, Zhang, Hui, Li, Guozhong, Wang, Dawei, Hu, Shengwei
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2016
Zugriff auf das übergeordnete Werk:	Asian-Australasian journal of animal sciences
Schlagworte:	Journal Article Myostatin Sheep Primary Fibroblasts Targeted Gene Editing Transcription Activator-like Effector Nucleases

Beschreibung
Zusammenfassung:	Myostatin (MSTN) is a secreted growth factor expressed in skeletal muscle and adipose tissue that negatively regulates skeletal muscle mass. Gene knockout of MSTN can result in increasing muscle mass in sheep. The objectives were to investigate whether myostatin gene can be edited in sheep by transcription activator-like effector nucleases (TALENs) in tandem with single-stranded DNA oligonucleotides (ssODNs). We designed a pair of TALENs to target a highly conserved sequence in the coding region of the sheep MSTN gene. The activity of the TALENs was verified by using luciferase single-strand annealing reporter assay in HEK 293T cell line. Co-transfection of TALENs and ssODNs oligonucleotides induced precise gene editing of myostatin gene in sheep primary fibroblasts. MSTN gene-edited cells were successfully used as nuclear donors for generating cloned embryos. TALENs combined with ssDNA oligonucleotides provide a useful approach for precise gene modification in livestock animals
Beschreibung:	Date Completed 08.03.2016 Date Revised 11.11.2023 published: Print-Electronic Citation Status PubMed-not-MEDLINE
ISSN:	1011-2367
DOI:	10.5713/ajas.15.0041