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231224s2013 xx |||||o 00| ||eng c |
| 024 |
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|a 10.1021/la400623s
|2 doi
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|a pubmed24n0761.xml
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|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Wang, Longgang
|e verfasserin
|4 aut
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| 245 |
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|a Reducing the cytotoxity of poly(amidoamine) dendrimers by modification of a single layer of carboxybetaine
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|c 2013
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 05.02.2014
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|a Date Revised 16.07.2013
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a The surface primary amines of generation five poly(amido amine) (G5 PAMAM) dendrimer were modified by different amounts of carboxybetaine acrylamide (CBAA). As a result, the fully modified molecules (CBAA-PAMAM-20, obtained from the 20:1 molar ratio of CBAA molecules to amino groups in modification solution) show excellent compatibility with protein and cells. CBAA-PAMAM-20 and fibrinogen (Fg) could coexist in solution without forming aggregation, indicating very weak interaction force between CBAA-PAMAM-20 and fibrinogen. CBAA-PAMAM-20 exhibits almost undetectable hemolytic activity, while other partially modified ones cause severe hemolysis and fibrinogen aggregation. Furthermore, the membrane of human umbilical vascular endothelial cell (HUVEC) remains intact after 24 h incubation with CBAA-PAMAM-20. The cytotoxicity assay of HUVEC cells and KB cells also showed that the CBAA-PAMAM-20 was not cytotoxic up to a 2 mg/mL concentration (>90% cell viability). In short, a thin compact layer of zwitterionic carboxybetaine could reduce the cytotoxicity of PAMAM through minimizing the interaction with protein and cell membranes, which suggest that the carboxybetaine-coated PAMAM could be a useful platform for biocompatible carriers to load contrast agents and drugs
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Biocompatible Materials
|2 NLM
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|a Dendrimers
|2 NLM
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|a PAMAM Starburst
|2 NLM
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|a Proteins
|2 NLM
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| 650 |
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|a Betaine
|2 NLM
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| 650 |
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|a 3SCV180C9W
|2 NLM
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| 700 |
1 |
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|a Wang, Zhen
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Ma, Guanglong
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Lin, Weifeng
|e verfasserin
|4 aut
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| 700 |
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|a Chen, Shengfu
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 29(2013), 28 vom: 16. Juli, Seite 8914-21
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
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|g volume:29
|g year:2013
|g number:28
|g day:16
|g month:07
|g pages:8914-21
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|u http://dx.doi.org/10.1021/la400623s
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