Cultured peripheral blood mast cells from chronic idiopathic urticaria patients spontaneously degranulate upon IgE sensitization : Relationship to expression of Syk and SHIP-2

Recently, signaling changes in the FcvarepsilonRI pathway involving inositol lipid phosphatases have been identified in the basophils of chronic idiopathic urticaria (CIU) subjects. Based on the profile of basophil FcvarepsilonRI-mediated histamine degranulation, we have segregated CIU subjects into...

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Veröffentlicht in:Clinical immunology (Orlando, Fla.). - 1999. - 132(2009), 3 vom: 01. Sept., Seite 342-8
1. Verfasser: Saini, Sarbjit S (VerfasserIn)
Weitere Verfasser: Paterniti, Miya, Vasagar, Kavitha, Gibbons, Scott P Jr, Sterba, Patricia M, Vonakis, Becky M
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2009
Zugriff auf das übergeordnete Werk:Clinical immunology (Orlando, Fla.)
Schlagworte:Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Antibodies Intracellular Signaling Peptides and Proteins Receptors, IgE Immunoglobulin E 37341-29-0 Histamine 820484N8I3 mehr... Protein-Tyrosine Kinases EC 2.7.10.1 Proto-Oncogene Proteins c-kit SYK protein, human EC 2.7.10.2 Syk Kinase Phosphoric Monoester Hydrolases EC 3.1.3.2 Inositol Polyphosphate 5-Phosphatases EC 3.1.3.56 INPP5D protein, human EC 3.1.3.86 INPPL1 protein, human Phosphatidylinositol-3,4,5-Trisphosphate 5-Phosphatases
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100 1 |a Saini, Sarbjit S  |e verfasserin  |4 aut 
245 1 0 |a Cultured peripheral blood mast cells from chronic idiopathic urticaria patients spontaneously degranulate upon IgE sensitization  |b Relationship to expression of Syk and SHIP-2 
264 1 |c 2009 
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520 |a Recently, signaling changes in the FcvarepsilonRI pathway involving inositol lipid phosphatases have been identified in the basophils of chronic idiopathic urticaria (CIU) subjects. Based on the profile of basophil FcvarepsilonRI-mediated histamine degranulation, we have segregated CIU subjects into two groups, CIU Responder (CIU R) or CIU Nonresponder (CIU NR). In the present study, we compared expression of SHIP-1, SHIP-2, and Syk protein to histamine release (HR) from mast cells (MC) cultured from the peripheral blood of CIU R, CIU NR, and normal subjects. The MC of CIU R donors contained significantly increased Syk and decreased SHIP-2 as compared to CIU NR (Syk: p=0.038, SHIP-2: p=0.038) and normals (Syk: p=0.042, SHIP-2: p=0.027). Spontaneous HR from CIU donors was increased two-fold compared to normals (p=0.04). In summary, our results suggest a possible predilection for urticarial MC to spontaneously degranulate upon IgE sensitization contributing to the increased pruritus associated with CIU 
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650 4 |a Research Support, Non-U.S. Gov't 
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650 7 |a Receptors, IgE  |2 NLM 
650 7 |a Immunoglobulin E  |2 NLM 
650 7 |a 37341-29-0  |2 NLM 
650 7 |a Histamine  |2 NLM 
650 7 |a 820484N8I3  |2 NLM 
650 7 |a Protein-Tyrosine Kinases  |2 NLM 
650 7 |a EC 2.7.10.1  |2 NLM 
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650 7 |a EC 2.7.10.1  |2 NLM 
650 7 |a SYK protein, human  |2 NLM 
650 7 |a EC 2.7.10.2  |2 NLM 
650 7 |a Syk Kinase  |2 NLM 
650 7 |a EC 2.7.10.2  |2 NLM 
650 7 |a Phosphoric Monoester Hydrolases  |2 NLM 
650 7 |a EC 3.1.3.2  |2 NLM 
650 7 |a Inositol Polyphosphate 5-Phosphatases  |2 NLM 
650 7 |a EC 3.1.3.56  |2 NLM 
650 7 |a INPP5D protein, human  |2 NLM 
650 7 |a EC 3.1.3.86  |2 NLM 
650 7 |a INPPL1 protein, human  |2 NLM 
650 7 |a EC 3.1.3.86  |2 NLM 
650 7 |a Phosphatidylinositol-3,4,5-Trisphosphate 5-Phosphatases  |2 NLM 
650 7 |a EC 3.1.3.86  |2 NLM 
700 1 |a Paterniti, Miya  |e verfasserin  |4 aut 
700 1 |a Vasagar, Kavitha  |e verfasserin  |4 aut 
700 1 |a Gibbons, Scott P  |c Jr  |e verfasserin  |4 aut 
700 1 |a Sterba, Patricia M  |e verfasserin  |4 aut 
700 1 |a Vonakis, Becky M  |e verfasserin  |4 aut 
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