Cultured peripheral blood mast cells from chronic idiopathic urticaria patients spontaneously degranulate upon IgE sensitization Relationship to expression of Syk and SHIP-2

Cultured peripheral blood mast cells from chronic idiopathic urticaria patients spontaneously degranulate upon IgE sensitization : Relationship to expression of Syk and SHIP-2

Recently, signaling changes in the FcvarepsilonRI pathway involving inositol lipid phosphatases have been identified in the basophils of chronic idiopathic urticaria (CIU) subjects. Based on the profile of basophil FcvarepsilonRI-mediated histamine degranulation, we have segregated CIU subjects into...

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Veröffentlicht in:Clinical immunology (Orlando, Fla.). - 1999. - 132(2009), 3 vom: 01. Sept., Seite 342-8
1. Verfasser: Saini, Sarbjit S (VerfasserIn)
Weitere Verfasser: Paterniti, Miya, Vasagar, Kavitha, Gibbons, Scott P Jr, Sterba, Patricia M, Vonakis, Becky M
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2009
Zugriff auf das übergeordnete Werk:Clinical immunology (Orlando, Fla.)
Schlagworte:Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Antibodies Intracellular Signaling Peptides and Proteins Receptors, IgE Immunoglobulin E 37341-29-0 Histamine 820484N8I3 mehr... Protein-Tyrosine Kinases EC 2.7.10.1 Proto-Oncogene Proteins c-kit SYK protein, human EC 2.7.10.2 Syk Kinase Phosphoric Monoester Hydrolases EC 3.1.3.2 Inositol Polyphosphate 5-Phosphatases EC 3.1.3.56 INPP5D protein, human EC 3.1.3.86 INPPL1 protein, human Phosphatidylinositol-3,4,5-Trisphosphate 5-Phosphatases
Beschreibung
Zusammenfassung:Recently, signaling changes in the FcvarepsilonRI pathway involving inositol lipid phosphatases have been identified in the basophils of chronic idiopathic urticaria (CIU) subjects. Based on the profile of basophil FcvarepsilonRI-mediated histamine degranulation, we have segregated CIU subjects into two groups, CIU Responder (CIU R) or CIU Nonresponder (CIU NR). In the present study, we compared expression of SHIP-1, SHIP-2, and Syk protein to histamine release (HR) from mast cells (MC) cultured from the peripheral blood of CIU R, CIU NR, and normal subjects. The MC of CIU R donors contained significantly increased Syk and decreased SHIP-2 as compared to CIU NR (Syk: p=0.038, SHIP-2: p=0.038) and normals (Syk: p=0.042, SHIP-2: p=0.027). Spontaneous HR from CIU donors was increased two-fold compared to normals (p=0.04). In summary, our results suggest a possible predilection for urticarial MC to spontaneously degranulate upon IgE sensitization contributing to the increased pruritus associated with CIU
Beschreibung:Date Completed 01.09.2009
Date Revised 20.10.2021
published: Print-Electronic
Citation Status MEDLINE
ISSN:1521-7035
DOI:10.1016/j.clim.2009.05.003