Coupled rolling circle amplification loop-mediated amplification for rapid detection of short DNA sequences
Circularizable oligonucleotide probes can detect short DNA sequences with single-base resolution at the site of ligation and can be amplified by rolling circle amplification (RCA) using strand displacing polymerases. A secondary amplification scheme was developed that uses the loop-mediated amplific...
| Veröffentlicht in: | BioTechniques. - 1988. - 45(2008), 3 vom: 01. Sept., Seite 275-80 |
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| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2008
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Journal Article Research Support, N.I.H., Extramural DNA Probes DNA, Circular DNA, Single-Stranded Oligonucleotide Probes DNA Ligases EC 6.5.1.- |
| Zusammenfassung: | Circularizable oligonucleotide probes can detect short DNA sequences with single-base resolution at the site of ligation and can be amplified by rolling circle amplification (RCA) using strand displacing polymerases. A secondary amplification scheme was developed that uses the loop-mediated amplification reaction concurrent with RCA to achieve rapid signal development from the starting circular molecules. This isothermal reaction was found to be significantly faster than the comparable hyperbranching amplification method and could detect 100 circular copies in less than 1 h |
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| Beschreibung: | Date Completed 14.11.2008 Date Revised 09.09.2008 published: Print Citation Status MEDLINE |
| ISSN: | 1940-9818 |
| DOI: | 10.2144/000112910 |