Coupled rolling circle amplification loop-mediated amplification for rapid detection of short DNA sequences
Circularizable oligonucleotide probes can detect short DNA sequences with single-base resolution at the site of ligation and can be amplified by rolling circle amplification (RCA) using strand displacing polymerases. A secondary amplification scheme was developed that uses the loop-mediated amplific...
Ausführliche Beschreibung
Bibliographische Detailangaben
| Veröffentlicht in: | BioTechniques. - 1988. - 45(2008), 3 vom: 01. Sept., Seite 275-80
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| 1. Verfasser: |
Marciniak, Jennifer
(VerfasserIn) |
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| Weitere Verfasser: |
Kummel, Andrew,
Esener, Sadik,
Heller, Michael,
Messmer, Bradley |
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| Format: | Online-Aufsatz
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| Sprache: | English |
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| Veröffentlicht: |
2008
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| Zugriff auf das übergeordnete Werk: | BioTechniques
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| Schlagworte: | Journal Article
Research Support, N.I.H., Extramural
DNA Probes
DNA, Circular
DNA, Single-Stranded
Oligonucleotide Probes
DNA Ligases
EC 6.5.1.- |
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