Selective screening of secretory vesicle-associated proteins for autoantigens in type 1 diabetes VAMP2 and NPY are new minor autoantigens

Selective screening of secretory vesicle-associated proteins for autoantigens in type 1 diabetes : VAMP2 and NPY are new minor autoantigens

The four major autoantigens (IA-2, IA-2 beta, GAD65 and insulin) of type 1 diabetes are all associated with dense core or synaptic vesicles. This raised the possibility that other secretory vesicle-associated proteins might be targets of the autoimmune response in type 1 diabetes. To test this hypot...

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Veröffentlicht in:Clinical immunology (Orlando, Fla.). - 1999. - 127(2008), 3 vom: 29. Juni, Seite 366-74
1. Verfasser: Hirai, Hiroki (VerfasserIn)
Weitere Verfasser: Miura, Junnosuke, Hu, Yafang, Larsson, Helena, Larsson, Karin, Lernmark, Ake, Ivarsson, Sten-A, Wu, Tianxia, Kingman, Albert, Tzioufas, Athanasios G, Notkins, Abner L
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2008
Zugriff auf das übergeordnete Werk:Clinical immunology (Orlando, Fla.)
Schlagworte:Journal Article Research Support, N.I.H., Intramural Autoantibodies Autoantigens Neuropeptide Y Vesicle-Associated Membrane Protein 2
Beschreibung
Zusammenfassung:The four major autoantigens (IA-2, IA-2 beta, GAD65 and insulin) of type 1 diabetes are all associated with dense core or synaptic vesicles. This raised the possibility that other secretory vesicle-associated proteins might be targets of the autoimmune response in type 1 diabetes. To test this hypothesis 56 proteins, two-thirds of which are associated with secretory vesicles, were prepared by in vitro transcription/translation and screened for autoantibodies by liquid phase radioimmunoprecipitation. Two secretory vesicle-associated proteins, VAMP2 and NPY, were identified as new minor autoantigens with 21% and 9%, respectively, of 200 type 1 diabetes sera reacting positively. These findings add support to the hypothesis that secretory vesicle-associated proteins are particularly important, but not the exclusive, targets of the autoimmune response in type 1 diabetes. Selective screening of the human proteome offers a useful approach for identifying new autoantigens in autoimmune diseases
Beschreibung:Date Completed 07.07.2008
Date Revised 20.10.2021
published: Print-Electronic
Citation Status MEDLINE
ISSN:1521-7035
DOI:10.1016/j.clim.2008.01.018