Intrinsic ability of GM+IL-4 but not Flt3L-induced rat dendritic cells to promote allogeneic T cell hyporesponsiveness

Intrinsic ability of GM+IL-4 but not Flt3L-induced rat dendritic cells to promote allogeneic T cell hyporesponsiveness

The influence of GM+IL-4 and Flt3 ligand (FL) on phenotype and function of BM-derived DC from Lewis rats was investigated. GM+IL-4-induced DC, despite expression of CD80/CD86, were less stimulatory than FL-induced DC that expressed low CD80/CD86 and were efficient stimulators of allogeneic T cells....

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Veröffentlicht in:Clinical immunology (Orlando, Fla.). - 1999. - 123(2007), 2 vom: 20. Mai, Seite 176-89
1. Verfasser: Taieb, Aurele (VerfasserIn)
Weitere Verfasser: Breitinger, Jeremy J, Unadkat, Jignesh V, Shufesky, William J, Morelli, Adrian E, Thomson, Angus W, Lee, W P Andrew, Feili-Hariri, Maryam
Format: Aufsatz
Sprache:English
Veröffentlicht: 2007
Zugriff auf das übergeordnete Werk:Clinical immunology (Orlando, Fla.)
Schlagworte:Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Antibodies, Monoclonal Antigens, CD Dinucleoside Phosphates Histocompatibility Antigens Isoantigens Lipopolysaccharides Membrane Proteins mehr... Receptors, Antigen, T-Cell flt3 ligand protein Interleukin-4 207137-56-2 cytidylyl-3'-5'-guanosine 2382-65-2 Granulocyte-Macrophage Colony-Stimulating Factor 83869-56-1 Interferons 9008-11-1
Beschreibung
Zusammenfassung:The influence of GM+IL-4 and Flt3 ligand (FL) on phenotype and function of BM-derived DC from Lewis rats was investigated. GM+IL-4-induced DC, despite expression of CD80/CD86, were less stimulatory than FL-induced DC that expressed low CD80/CD86 and were efficient stimulators of allogeneic T cells. GM+IL-4 DC were CD11b+ OX62lo, whereas FL DC were CD11blo OX62+. Following activation, GM+IL-4 DC produced IL-10 and IL-6, but no IL-12p70, and were resistant to further maturation. FL DC produced IL-12p70, IFN-alpha/beta, IL-10 and IL-6 and underwent maturation. Repeated stimulation of T cells with GM+IL-4 DC inhibited proliferation, cytokine production and induced early T cell apoptosis. FL DC-activated T cells produced large amounts of IFN-gamma/IL-10 and exhibited late T cell apoptosis/necrosis. In vivo, GM+IL-4 DC induced alloAg-specific hyporesponsiveness following T cell restimulation. These results demonstrate that GM+IL-4 DC display intrinsic regulatory properties, inducing passive-cell-death in T cells with potential for inactivation/regulation of alloreactive T cells in transplantation
Beschreibung:Date Completed 26.06.2007
Date Revised 03.12.2007
published: Print-Electronic
Citation Status MEDLINE
ISSN:1521-7035