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20231223052020.0 |
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231223s2004 xx ||||| 00| ||eng c |
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|a pubmed24n0499.xml
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|a (DE-627)NLM149574894
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|a (NLM)15274593
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Graveland-Bikker, Johanna F
|e verfasserin
|4 aut
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|a Influence of calcium on the self-assembly of partially hydrolyzed alpha-lactalbumin
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|c 2004
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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|a Band
|b nc
|2 rdacarrier
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|a Date Completed 22.05.2006
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|a Date Revised 21.11.2013
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|a published: Print
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|a Citation Status MEDLINE
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|a Self-assembly of alpha-lactalbumin after partial hydrolysis by a protease from Bacillus licheniformis can result in nanotubular structures, which show many similarities to microtubules. Calcium plays a crucial role in this process. The objective of this investigation was to study the role of calcium in more detail. The kinetics of the hydrolysis step and the self-assembly step were monitored by respectively liquid chromatography-mass spectrometry and dynamic light scattering. The microstructure of the gels finally formed was investigated by transmission electron microscopy. This investigation demonstrates that calcium accelerated the kinetics of the self-assembly, but it had no effect on the hydrolysis kinetics. As a result of the accelerated self-assembly kinetics at a high calcium concentration, the time of gelation decreased as well. A minimum concentration of calcium needed to obtain the tubular alpha-lactalbumin structures was determined. Below R = 1.5 (mole calcium/mole alpha-lactalbumin), turbid gels with randomlike structure were obtained. Between R = 1.5 and R = 6, translucent gels with a fine stranded network of tubules were formed, while higher calcium concentrations had a negative effect on the tubule formation, resulting in amorphous structures. The optimum calcium concentration for alpha-lactalbumin nanotube formation seemed to be around R = 3
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Lactalbumin
|2 NLM
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|a 9013-90-5
|2 NLM
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|a Calcium
|2 NLM
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|a SY7Q814VUP
|2 NLM
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1 |
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|a Ipsen, Richard
|e verfasserin
|4 aut
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1 |
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|a Otte, Jeanette
|e verfasserin
|4 aut
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| 700 |
1 |
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|a de Kruif, Cornelis G
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1999
|g 20(2004), 16 vom: 03. Aug., Seite 6841-6
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
1 |
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|g volume:20
|g year:2004
|g number:16
|g day:03
|g month:08
|g pages:6841-6
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|d 20
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|h 6841-6
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