Influence of calcium on the self-assembly of partially hydrolyzed alpha-lactalbumin
Self-assembly of alpha-lactalbumin after partial hydrolysis by a protease from Bacillus licheniformis can result in nanotubular structures, which show many similarities to microtubules. Calcium plays a crucial role in this process. The objective of this investigation was to study the role of calcium...
| Veröffentlicht in: | Langmuir : the ACS journal of surfaces and colloids. - 1999. - 20(2004), 16 vom: 03. Aug., Seite 6841-6 |
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| Weitere Verfasser: | , , |
| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2004
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| Zugriff auf das übergeordnete Werk: | Langmuir : the ACS journal of surfaces and colloids |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Lactalbumin 9013-90-5 Calcium SY7Q814VUP |
| Zusammenfassung: | Self-assembly of alpha-lactalbumin after partial hydrolysis by a protease from Bacillus licheniformis can result in nanotubular structures, which show many similarities to microtubules. Calcium plays a crucial role in this process. The objective of this investigation was to study the role of calcium in more detail. The kinetics of the hydrolysis step and the self-assembly step were monitored by respectively liquid chromatography-mass spectrometry and dynamic light scattering. The microstructure of the gels finally formed was investigated by transmission electron microscopy. This investigation demonstrates that calcium accelerated the kinetics of the self-assembly, but it had no effect on the hydrolysis kinetics. As a result of the accelerated self-assembly kinetics at a high calcium concentration, the time of gelation decreased as well. A minimum concentration of calcium needed to obtain the tubular alpha-lactalbumin structures was determined. Below R = 1.5 (mole calcium/mole alpha-lactalbumin), turbid gels with randomlike structure were obtained. Between R = 1.5 and R = 6, translucent gels with a fine stranded network of tubules were formed, while higher calcium concentrations had a negative effect on the tubule formation, resulting in amorphous structures. The optimum calcium concentration for alpha-lactalbumin nanotube formation seemed to be around R = 3 |
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| Beschreibung: | Date Completed 22.05.2006 Date Revised 21.11.2013 published: Print Citation Status MEDLINE |
| ISSN: | 1520-5827 |