Determination of methylation site of DNA-methyltransferase NlaX by a hybrid method
Using a new method based on a combination of bisulfite reaction, the repair enzyme uracil-DNA glycosylase, and synthetic oligodeoxyribonucleotides, the methylation site of DNA-methyltransferase NlaX (M.NlaX) from Neisseria lactamica was established to be the inner cytosine in the double-stranded pen...
Publié dans: | BioTechniques. - 1993. - 33(2002), 3 vom: 11. Sept., Seite 526-31 |
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Auteur principal: | |
Autres auteurs: | , , , , |
Format: | Article |
Langue: | English |
Publié: |
2002
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Accès à la collection: | BioTechniques |
Sujets: | Evaluation Study Research Support, Non-U.S. Gov't Technical Report Journal Article Cross-Linking Reagents DNA, Bacterial Sulfites DNA Modification Methylases EC 2.1.1.- endodeoxyribonuclease NlaIII plus... |