Determination of methylation site of DNA-methyltransferase NlaX by a hybrid method

Determination of methylation site of DNA-methyltransferase NlaX by a hybrid method

Using a new method based on a combination of bisulfite reaction, the repair enzyme uracil-DNA glycosylase, and synthetic oligodeoxyribonucleotides, the methylation site of DNA-methyltransferase NlaX (M.NlaX) from Neisseria lactamica was established to be the inner cytosine in the double-stranded pen...

Ausführliche Beschreibung

Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1993. - 33(2002), 3 vom: 11. Sept., Seite 526-31
1. Verfasser: Kubareva, E A (VerfasserIn)
Weitere Verfasser: Walter, J, Karyagina, A S, Vorob'eva, O V, Lau, P C K, Trautner, T
Format: Aufsatz
Sprache:English
Veröffentlicht: 2002
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Evaluation Study Research Support, Non-U.S. Gov't Technical Report Journal Article Cross-Linking Reagents DNA, Bacterial Sulfites DNA Modification Methylases EC 2.1.1.- endodeoxyribonuclease NlaIII mehr... EC 3.1.21.- endodeoxyribonuclease NlaIV Deoxyribonucleases, Type II Site-Specific EC 3.1.21.4 DNA Glycosylases EC 3.2.2.- N-Glycosyl Hydrolases Uracil-DNA Glycosidase