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231222s2002 xx ||||| 00| ||eng c |
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|a (DE-627)NLM120958368
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|a (NLM)12238762
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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1 |
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|a Kubareva, E A
|e verfasserin
|4 aut
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|a Determination of methylation site of DNA-methyltransferase NlaX by a hybrid method
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|c 2002
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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|a Band
|b nc
|2 rdacarrier
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|a Date Completed 07.05.2003
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|a Date Revised 10.12.2019
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|a published: Print
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|a Citation Status MEDLINE
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|a Using a new method based on a combination of bisulfite reaction, the repair enzyme uracil-DNA glycosylase, and synthetic oligodeoxyribonucleotides, the methylation site of DNA-methyltransferase NlaX (M.NlaX) from Neisseria lactamica was established to be the inner cytosine in the double-stranded pentanucleotide recognition sequence 5'-CCNGG-3' (where N = any nucleoside). 5-Methylcytosine (m5C) type modification by M-N1aX was confirmed by the use of oligonucleotide substrates that contain 5-fluoro-2'-deoxycytidine
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|a Evaluation Study
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|a Research Support, Non-U.S. Gov't
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|a Technical Report
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|a Journal Article
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|a Cross-Linking Reagents
|2 NLM
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|a DNA, Bacterial
|2 NLM
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|a Sulfites
|2 NLM
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|a DNA Modification Methylases
|2 NLM
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|a EC 2.1.1.-
|2 NLM
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|a endodeoxyribonuclease NlaIII
|2 NLM
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|a EC 3.1.21.-
|2 NLM
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|a endodeoxyribonuclease NlaIV
|2 NLM
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|a EC 3.1.21.-
|2 NLM
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|a Deoxyribonucleases, Type II Site-Specific
|2 NLM
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|a EC 3.1.21.4
|2 NLM
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|a DNA Glycosylases
|2 NLM
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|a EC 3.2.2.-
|2 NLM
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|a N-Glycosyl Hydrolases
|2 NLM
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|a EC 3.2.2.-
|2 NLM
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|a Uracil-DNA Glycosidase
|2 NLM
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7 |
|a EC 3.2.2.-
|2 NLM
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1 |
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|a Walter, J
|e verfasserin
|4 aut
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700 |
1 |
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|a Karyagina, A S
|e verfasserin
|4 aut
|
700 |
1 |
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|a Vorob'eva, O V
|e verfasserin
|4 aut
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1 |
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|a Lau, P C K
|e verfasserin
|4 aut
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1 |
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|a Trautner, T
|e verfasserin
|4 aut
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773 |
0 |
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|i Enthalten in
|t BioTechniques
|d 1993
|g 33(2002), 3 vom: 11. Sept., Seite 526-31
|w (DE-627)NLM012627046
|x 0736-6205
|7 nnns
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1 |
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|g volume:33
|g year:2002
|g number:3
|g day:11
|g month:09
|g pages:526-31
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|d 33
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