Determination of methylation site of DNA-methyltransferase NlaX by a hybrid method

Using a new method based on a combination of bisulfite reaction, the repair enzyme uracil-DNA glycosylase, and synthetic oligodeoxyribonucleotides, the methylation site of DNA-methyltransferase NlaX (M.NlaX) from Neisseria lactamica was established to be the inner cytosine in the double-stranded pen...

Description complète

Détails bibliographiques
Publié dans:BioTechniques. - 1993. - 33(2002), 3 vom: 11. Sept., Seite 526-31
Auteur principal: Kubareva, E A (Auteur)
Autres auteurs: Walter, J, Karyagina, A S, Vorob'eva, O V, Lau, P C K, Trautner, T
Format: Article
Langue:English
Publié: 2002
Accès à la collection:BioTechniques
Sujets:Evaluation Study Research Support, Non-U.S. Gov't Technical Report Journal Article Cross-Linking Reagents DNA, Bacterial Sulfites DNA Modification Methylases EC 2.1.1.- endodeoxyribonuclease NlaIII plus... EC 3.1.21.- endodeoxyribonuclease NlaIV Deoxyribonucleases, Type II Site-Specific EC 3.1.21.4 DNA Glycosylases EC 3.2.2.- N-Glycosyl Hydrolases Uracil-DNA Glycosidase
LEADER 01000caa a22002652 4500
001 NLM120958368
003 DE-627
005 20250203115619.0
007 tu
008 231222s2002 xx ||||| 00| ||eng c
028 5 2 |a pubmed25n0403.xml 
035 |a (DE-627)NLM120958368 
035 |a (NLM)12238762 
040 |a DE-627  |b ger  |c DE-627  |e rakwb 
041 |a eng 
100 1 |a Kubareva, E A  |e verfasserin  |4 aut 
245 1 0 |a Determination of methylation site of DNA-methyltransferase NlaX by a hybrid method 
264 1 |c 2002 
336 |a Text  |b txt  |2 rdacontent 
337 |a ohne Hilfsmittel zu benutzen  |b n  |2 rdamedia 
338 |a Band  |b nc  |2 rdacarrier 
500 |a Date Completed 07.05.2003 
500 |a Date Revised 10.12.2019 
500 |a published: Print 
500 |a Citation Status MEDLINE 
520 |a Using a new method based on a combination of bisulfite reaction, the repair enzyme uracil-DNA glycosylase, and synthetic oligodeoxyribonucleotides, the methylation site of DNA-methyltransferase NlaX (M.NlaX) from Neisseria lactamica was established to be the inner cytosine in the double-stranded pentanucleotide recognition sequence 5'-CCNGG-3' (where N = any nucleoside). 5-Methylcytosine (m5C) type modification by M-N1aX was confirmed by the use of oligonucleotide substrates that contain 5-fluoro-2'-deoxycytidine 
650 4 |a Evaluation Study 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a Technical Report 
650 4 |a Journal Article 
650 7 |a Cross-Linking Reagents  |2 NLM 
650 7 |a DNA, Bacterial  |2 NLM 
650 7 |a Sulfites  |2 NLM 
650 7 |a DNA Modification Methylases  |2 NLM 
650 7 |a EC 2.1.1.-  |2 NLM 
650 7 |a endodeoxyribonuclease NlaIII  |2 NLM 
650 7 |a EC 3.1.21.-  |2 NLM 
650 7 |a endodeoxyribonuclease NlaIV  |2 NLM 
650 7 |a EC 3.1.21.-  |2 NLM 
650 7 |a Deoxyribonucleases, Type II Site-Specific  |2 NLM 
650 7 |a EC 3.1.21.4  |2 NLM 
650 7 |a DNA Glycosylases  |2 NLM 
650 7 |a EC 3.2.2.-  |2 NLM 
650 7 |a N-Glycosyl Hydrolases  |2 NLM 
650 7 |a EC 3.2.2.-  |2 NLM 
650 7 |a Uracil-DNA Glycosidase  |2 NLM 
650 7 |a EC 3.2.2.-  |2 NLM 
700 1 |a Walter, J  |e verfasserin  |4 aut 
700 1 |a Karyagina, A S  |e verfasserin  |4 aut 
700 1 |a Vorob'eva, O V  |e verfasserin  |4 aut 
700 1 |a Lau, P C K  |e verfasserin  |4 aut 
700 1 |a Trautner, T  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t BioTechniques  |d 1993  |g 33(2002), 3 vom: 11. Sept., Seite 526-31  |w (DE-627)NLM012627046  |x 0736-6205  |7 nnns 
773 1 8 |g volume:33  |g year:2002  |g number:3  |g day:11  |g month:09  |g pages:526-31 
912 |a GBV_USEFLAG_A 
912 |a SYSFLAG_A 
912 |a GBV_NLM 
912 |a GBV_ILN_21 
912 |a GBV_ILN_22 
912 |a GBV_ILN_39 
912 |a GBV_ILN_40 
912 |a GBV_ILN_50 
912 |a GBV_ILN_60 
912 |a GBV_ILN_62 
912 |a GBV_ILN_65 
912 |a GBV_ILN_70 
912 |a GBV_ILN_99 
912 |a GBV_ILN_121 
912 |a GBV_ILN_130 
912 |a GBV_ILN_227 
912 |a GBV_ILN_350 
912 |a GBV_ILN_618 
912 |a GBV_ILN_640 
912 |a GBV_ILN_754 
912 |a GBV_ILN_2001 
912 |a GBV_ILN_2002 
912 |a GBV_ILN_2003 
912 |a GBV_ILN_2005 
912 |a GBV_ILN_2006 
912 |a GBV_ILN_2007 
912 |a GBV_ILN_2008 
912 |a GBV_ILN_2009 
912 |a GBV_ILN_2010 
912 |a GBV_ILN_2012 
912 |a GBV_ILN_2015 
912 |a GBV_ILN_2018 
912 |a GBV_ILN_2023 
912 |a GBV_ILN_2035 
912 |a GBV_ILN_2040 
912 |a GBV_ILN_2060 
912 |a GBV_ILN_2099 
912 |a GBV_ILN_2105 
912 |a GBV_ILN_2121 
912 |a GBV_ILN_2470 
951 |a AR 
952 |d 33  |j 2002  |e 3  |b 11  |c 09  |h 526-31