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231222s1996 xx ||||| 00| ||eng c |
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|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Zeng, M
|e verfasserin
|4 aut
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| 245 |
1 |
0 |
|a Cloning strategies using a third irrelevant enzyme site (TIES) to overcome certain cloning problems
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| 264 |
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1 |
|c 1996
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 25.10.1996
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| 500 |
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|a Date Revised 28.09.2018
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|a published: Print
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|a Citation Status MEDLINE
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| 520 |
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|a We describe a cloning strategy, third irrelevant enzyme site (TIES), based on three-component assembly ligation reactions to overcome seven types of problematical cloning reactions. Implementation of the TIES strategy has made it possible to obtain the desired recombinant where the corresponding approach using conventional recombinant DNA methodologies and two-component ligations failed to give the desired product
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| 650 |
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4 |
|a Comparative Study
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| 650 |
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4 |
|a Research Support, U.S. Gov't, P.H.S.
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| 650 |
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4 |
|a Technical Report
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| 650 |
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4 |
|a Journal Article
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| 650 |
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7 |
|a DNA, Recombinant
|2 NLM
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| 650 |
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7 |
|a DNA Restriction Enzymes
|2 NLM
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| 650 |
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7 |
|a EC 3.1.21.-
|2 NLM
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| 700 |
1 |
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|a Huang, B R
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Lapeyre, J N
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t BioTechniques
|d 1993
|g 20(1996), 1 vom: 05. Jan., Seite 68-72, 74, 76 passim
|w (DE-627)NLM012627046
|x 0736-6205
|7 nnns
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| 773 |
1 |
8 |
|g volume:20
|g year:1996
|g number:1
|g day:05
|g month:01
|g pages:68-72, 74, 76 passim
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