Cloning strategies using a third irrelevant enzyme site (TIES) to overcome certain cloning problems
We describe a cloning strategy, third irrelevant enzyme site (TIES), based on three-component assembly ligation reactions to overcome seven types of problematical cloning reactions. Implementation of the TIES strategy has made it possible to obtain the desired recombinant where the corresponding app...
| Veröffentlicht in: | BioTechniques. - 1993. - 20(1996), 1 vom: 05. Jan., Seite 68-72, 74, 76 passim |
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| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
1996
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Comparative Study Research Support, U.S. Gov't, P.H.S. Technical Report Journal Article DNA, Recombinant DNA Restriction Enzymes EC 3.1.21.- |