Cloning strategies using a third irrelevant enzyme site (TIES) to overcome certain cloning problems
We describe a cloning strategy, third irrelevant enzyme site (TIES), based on three-component assembly ligation reactions to overcome seven types of problematical cloning reactions. Implementation of the TIES strategy has made it possible to obtain the desired recombinant where the corresponding app...
| Veröffentlicht in: | BioTechniques. - 1993. - 20(1996), 1 vom: 05. Jan., Seite 68-72, 74, 76 passim |
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| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
1996
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Comparative Study Research Support, U.S. Gov't, P.H.S. Technical Report Journal Article DNA, Recombinant DNA Restriction Enzymes EC 3.1.21.- |
| Zusammenfassung: | We describe a cloning strategy, third irrelevant enzyme site (TIES), based on three-component assembly ligation reactions to overcome seven types of problematical cloning reactions. Implementation of the TIES strategy has made it possible to obtain the desired recombinant where the corresponding approach using conventional recombinant DNA methodologies and two-component ligations failed to give the desired product |
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| Beschreibung: | Date Completed 25.10.1996 Date Revised 28.09.2018 published: Print Citation Status MEDLINE |
| ISSN: | 0736-6205 |