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20250129121232.0 |
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231222s1995 xx ||||| 00| ||eng c |
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|a pubmed25n0256.xml
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|a (DE-627)NLM076787788
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|a (NLM)7702842
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Meador, J W
|c 3rd
|e verfasserin
|4 aut
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| 245 |
1 |
0 |
|a pTRIPLEscript
|b a novel cloning vector for generating in vitro transcripts from tandem promoters for SP6, T7 and T3 RNA polymerase
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| 264 |
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1 |
|c 1995
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 10.05.1995
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|a Date Revised 21.11.2008
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|a published: Print
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|a Citation Status MEDLINE
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|a We have constructed a family of novel in vitro transcription vectors in which functional T3, T7 and SP6 RNA polymerase promoters are arranged in tandem and directed towards a multiple cloning site. This prototype vector, named pTRIPLEscript, permits the transcription of one strand of a DNA insert by any of the three commonly used bacteriophage RNA polymerases with no apparent cross talk, i.e., use of the wrong promoter sequence. The vector has two main uses: (i) to clone probe sequences that will be distributed to many laboratories, allowing the use of the most convenient RNA polymerase; and (ii) to circumvent the problem of RNA polymerase-dependent premature termination
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| 650 |
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4 |
|a Journal Article
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| 650 |
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7 |
|a Viral Proteins
|2 NLM
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| 650 |
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7 |
|a RNA polymerase SP6
|2 NLM
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| 650 |
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7 |
|a EC 2.7.7.-
|2 NLM
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| 650 |
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7 |
|a bacteriophage T3 RNA polymerase
|2 NLM
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| 650 |
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7 |
|a EC 2.7.7.-
|2 NLM
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| 650 |
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7 |
|a bacteriophage T7 RNA polymerase
|2 NLM
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| 650 |
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7 |
|a EC 2.7.7.-
|2 NLM
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| 650 |
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7 |
|a DNA-Directed RNA Polymerases
|2 NLM
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| 650 |
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7 |
|a EC 2.7.7.6
|2 NLM
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| 700 |
1 |
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|a McElroy, H E
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Pasloske, B L
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Milburn, S C
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Winkler, M M
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t BioTechniques
|d 1988
|g 18(1995), 1 vom: 01. Jan., Seite 152-7,
|w (DE-627)NLM012627046
|x 0736-6205
|7 nnns
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| 773 |
1 |
8 |
|g volume:18
|g year:1995
|g number:1
|g day:01
|g month:01
|g pages:152-7,
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|d 18
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