pTRIPLEscript : a novel cloning vector for generating in vitro transcripts from tandem promoters for SP6, T7 and T3 RNA polymerase

We have constructed a family of novel in vitro transcription vectors in which functional T3, T7 and SP6 RNA polymerase promoters are arranged in tandem and directed towards a multiple cloning site. This prototype vector, named pTRIPLEscript, permits the transcription of one strand of a DNA insert by...

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Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1988. - 18(1995), 1 vom: 01. Jan., Seite 152-7,
1. Verfasser: Meador, J W 3rd (VerfasserIn)
Weitere Verfasser: McElroy, H E, Pasloske, B L, Milburn, S C, Winkler, M M
Format: Aufsatz
Sprache:English
Veröffentlicht: 1995
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article Viral Proteins RNA polymerase SP6 EC 2.7.7.- bacteriophage T3 RNA polymerase bacteriophage T7 RNA polymerase DNA-Directed RNA Polymerases EC 2.7.7.6