pTRIPLEscript : a novel cloning vector for generating in vitro transcripts from tandem promoters for SP6, T7 and T3 RNA polymerase
We have constructed a family of novel in vitro transcription vectors in which functional T3, T7 and SP6 RNA polymerase promoters are arranged in tandem and directed towards a multiple cloning site. This prototype vector, named pTRIPLEscript, permits the transcription of one strand of a DNA insert by...
| Veröffentlicht in: | BioTechniques. - 1988. - 18(1995), 1 vom: 01. Jan., Seite 152-7, |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , , |
| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
1995
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Journal Article Viral Proteins RNA polymerase SP6 EC 2.7.7.- bacteriophage T3 RNA polymerase bacteriophage T7 RNA polymerase DNA-Directed RNA Polymerases EC 2.7.7.6 |
| Zusammenfassung: | We have constructed a family of novel in vitro transcription vectors in which functional T3, T7 and SP6 RNA polymerase promoters are arranged in tandem and directed towards a multiple cloning site. This prototype vector, named pTRIPLEscript, permits the transcription of one strand of a DNA insert by any of the three commonly used bacteriophage RNA polymerases with no apparent cross talk, i.e., use of the wrong promoter sequence. The vector has two main uses: (i) to clone probe sequences that will be distributed to many laboratories, allowing the use of the most convenient RNA polymerase; and (ii) to circumvent the problem of RNA polymerase-dependent premature termination |
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| Beschreibung: | Date Completed 10.05.1995 Date Revised 21.11.2008 published: Print Citation Status MEDLINE |
| ISSN: | 0736-6205 |