Evolutionary and Functional Significance of ShPP2C1 in the Parasitic Life Strategy of Striga

© The Author(s) 2025. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For commercial re-use, please contact reprintsoup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink serv...

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Veröffentlicht in:Journal of experimental botany. - 1985. - (2025) vom: 18. Sept.
1. Verfasser: Katagiri, Sotaro (VerfasserIn)
Weitere Verfasser: Fukuhara, Daisuke, Fujiyama, Keisuke, Fujioka, Hijiri, Sugimoto, Yukihiro, Okamoto, Masanori
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2025
Zugriff auf das übergeordnete Werk:Journal of experimental botany
Schlagworte:Journal Article Striga Abscisic acid Arabidopsis Molecular biology Parasitic plant Phylogenetic analysis
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520 |a Parasitic plants have evolved specialized mechanisms to extract water and nutrients from their hosts, often causing severe agricultural losses. Striga hermonthica, a member of the Orobanchaceae family, exhibits a unique adaptation in the abscisic acid (ABA) signaling pathway, which enhances its growth efficiency during parasitism. Striga hermonthica protein phosphatase 2C (ShPP2C1) is a negative regulator of ABA signaling but, unlike typical PP2Cs, is not inhibited by ABA receptors (PYLs). Because of ShPP2C1, Striga hermonthica shows low ABA sensitivity and high transpiration, facilitating resource uptake from the host. To determine whether similar PP2Cs are conserved in other parasitic Orobanchaceae, we performed a phylogenetic analysis using public sequence data, revealing that ShPP2C1 is unique to the genus Striga genus. Furthermore, in vitro phosphatase assay revealed specific amino acid residues responsible for disrupting the inhibition by PYLs. Structural comparisons between ShPP2C1 and Arabidopsis thaliana ABI1 showed that the lack of interaction to PYLs is due to steric hindrance from a combination of amino acid substitutions. These findings provide new insight into the molecular basis of Striga's parasitic strategy 
650 4 |a Journal Article 
650 4 |a Striga 
650 4 |a Abscisic acid 
650 4 |a Arabidopsis 
650 4 |a Molecular biology 
650 4 |a Parasitic plant 
650 4 |a Phylogenetic analysis 
700 1 |a Fukuhara, Daisuke  |e verfasserin  |4 aut 
700 1 |a Fujiyama, Keisuke  |e verfasserin  |4 aut 
700 1 |a Fujioka, Hijiri  |e verfasserin  |4 aut 
700 1 |a Sugimoto, Yukihiro  |e verfasserin  |4 aut 
700 1 |a Okamoto, Masanori  |e verfasserin  |4 aut 
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