Optimizing chromosome yield : a comparative analysis of harvesting, preparation and waste recovery methods

In mitotic chromosome preparation, it is crucial to maximize chromosome yield for downstream cytogenetic analysis. Using HeLa cells as a model adherent cell, we assessed and compared the recovery of chromosomes from the entire process as well as the fraction of chromosomes that would generally becom...

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Publié dans:BioTechniques. - 1991. - 77(2025), 5-6 vom: 02. Mai, Seite 245-256
Auteur principal: Berger, Sarah L (Auteur)
Autres auteurs: Phengchat, Rinyaporn, Botchway, Stanley W, Yusuf, Mohammed
Format: Article en ligne
Langue:English
Publié: 2025
Accès à la collection:BioTechniques
Sujets:Journal Article Comparative Study chromosomes methanol acetic acid mitotic mitotic inhibitors polyamine Polyamines Nocodazole SH1WY3R615 plus... Trypsin EC 3.4.21.4