Optimizing chromosome yield : a comparative analysis of harvesting, preparation and waste recovery methods

In mitotic chromosome preparation, it is crucial to maximize chromosome yield for downstream cytogenetic analysis. Using HeLa cells as a model adherent cell, we assessed and compared the recovery of chromosomes from the entire process as well as the fraction of chromosomes that would generally becom...

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Veröffentlicht in:BioTechniques. - 1991. - 77(2025), 5-6 vom: 02. Mai, Seite 245-256
1. Verfasser: Berger, Sarah L (VerfasserIn)
Weitere Verfasser: Phengchat, Rinyaporn, Botchway, Stanley W, Yusuf, Mohammed
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2025
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article Comparative Study chromosomes methanol acetic acid mitotic mitotic inhibitors polyamine Polyamines Nocodazole SH1WY3R615 mehr... Trypsin EC 3.4.21.4