Natural variation in the promoter of TaHY5 enhances anthocyanin biosynthesis via upregulated transcription of TaF3H and TaANS in purple grain wheat (Triticum aestivum L.)
Copyright © 2025 Elsevier Masson SAS. All rights reserved.
| Publié dans: | Plant physiology and biochemistry : PPB. - 1991. - 228(2025) vom: 14. Juli, Seite 110246 |
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| Auteur principal: | |
| Autres auteurs: | , , , , , , , , |
| Format: | Article en ligne |
| Langue: | English |
| Publié: |
2025
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| Accès à la collection: | Plant physiology and biochemistry : PPB |
| Sujets: | Journal Article Anthocyanin Purple grain wheat TaHY5 Transcription factor |
| Résumé: | Copyright © 2025 Elsevier Masson SAS. All rights reserved. Purple grain wheat (Triticum aestivum L.) has attracted increasing attention owing to its high anthocyanin content that is beneficial to human health; however, the molecular mechanisms governing its pigmentation remain poorly characterized. In this study, two anthocyanin-related structural genes, TaF3H (TraesCS1D02G319700) and TaANS (TraesCS6D02G004300), and one transcription factor (TF) gene, TaHY5 (TraesCS6B02G209600), were significantly upregulated in purple grain wheat. Functional characterization of TaHY5 revealed its nuclear localization and transcription activity, validating its predicted role as a transcriptional regulator. The ectopic expression of TaHY5 in tobacco (Nicotiana benthamiana) significantly upregulated endogenous anthocyanin biosynthetic genes NtF3H and NtANS, and promoted anthocyanin accumulation. Moreover, transient silencing of TaHY5 in purple grain wheat significantly downregulated the expression of TaF3H and TaANS, decreasing the anthocyanin content in the coleoptiles. Yeast one-hybrid (Y1H) assays reflected that TaHY5 directly binds to the ACE box (with the ACGT motif) on the TaF3H promoter and to the G-box (with the CACGTG motif) on the TaANS promoter, activating the transcription of the corresponding genes. Furthermore, the purple-grain variety of wheat contains more copies of light-responsive ACE-motif within the cis-acting element of TaHY5 promoter compared with the white grain wheat; this natural variation results in enhanced transcriptional activity of TaHY5. In conclusion, our findings established that TaHY5 serves as a key regulator of anthocyanin biosynthesis in purple grain wheat, directly activating TaF3H and TaANS to drive anthocyanin biosynthesis |
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| Description: | Date Revised 17.07.2025 published: Print-Electronic Citation Status Publisher |
| ISSN: | 1873-2690 |
| DOI: | 10.1016/j.plaphy.2025.110246 |