HFR1/PIF module balances chlorophyll biosynthesis to promote greening during de-etiolation in Arabidopsis
© The Author(s) 2025. Published by Oxford University Press on behalf of Society for Experimental Biology. All rights reserved. For commercial re-use, please contact reprintsoup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service...
| Veröffentlicht in: | Journal of experimental botany. - 1985. - (2025) vom: 19. Mai |
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| Weitere Verfasser: | , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2025
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| Zugriff auf das übergeordnete Werk: | Journal of experimental botany |
| Schlagworte: | Journal Article Chlorophyll biosynthesis HFR1 Oxidative stress PIF Pchlide Singlet oxygen de-etiolation |
| Zusammenfassung: | © The Author(s) 2025. Published by Oxford University Press on behalf of Society for Experimental Biology. All rights reserved. For commercial re-use, please contact reprintsoup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact journals.permissions@oup.com. During de-etiolation, dark-grown seedlings are exposed to light, which triggers chlorophyll biosynthesis and greening of the cotyledons. LONG HYPOCOTYL IN FAR-RED 1 (HFR1) is known to interact with PHYTOCHROME INTERACTING FACTORs (PIFs) to regulate many light-mediated developmental processes in Arabidopsis. Here, we found that seedlings overexpressing HFR1 [HFR1(ΔN)-OE] showed photo-oxidative bleaching and reduced greening during de-etiolation, which is similar to pif1-1. To elucidate the role of HFR1 in regulating de-etiolation and greening, transcriptome analyses were performed on seedlings of hfr1-5, HFR1(ΔN)-OE, and pif mutants under 0, 1, and 6 h of de-etiolation. We found that PIFs and HFR1 exert opposing regulation of genes related to chlorophyll biosynthesis, photosynthesis, and oxidative stress during de-etiolation. Importantly, HFR1 promoted the expression of genes related to antioxidant activity and inhibition of programmed cell death, along with reduced protochlorophyllide (Pchlide) accumulation, potentially explaining the attenuated photobleaching observed in HFR1(ΔN)-OE, as compared to pifq. Further analysis of the tetrapyrrole biosynthetic pathway revealed that gene regulation by HFR1 and PIFs at 6 h de-etiolation coincides with their photo-oxidative phenotypes. While HFR1 suppresses the tetrapyrrole biosynthesis genes, PIFs promote their expression, which influences the accumulation of protochlorophyllide and burst of singlet oxygen during de-etiolation, thereby causing photobleaching |
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| Beschreibung: | Date Revised 19.05.2025 published: Print-Electronic Citation Status Publisher |
| ISSN: | 1460-2431 |
| DOI: | 10.1093/jxb/eraf217 |