Glucosinolates can act as signals to modulate intercellular trafficking via plasmodesmata

Glucosinolates can act as signals to modulate intercellular trafficking via plasmodesmata

© 2025 The Author(s). New Phytologist © 2025 New Phytologist Foundation.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 246(2025), 3 vom: 08. Mai, Seite 1163-1182
1. Verfasser: Fernandez, Jessica C (VerfasserIn)
Weitere Verfasser: Azim, Mohammad F, Adams, Nicole, Strong, Morgan, Piya, Sarbottam, Xu, Min, Brunkard, Jacob O, Hewezi, Tarek, Sams, Carl E, Burch-Smith, Tessa M
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2025
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Arabidopsis auxin callose glucosinolates intercellular trafficking myrosinase plasmodesmata signaling Glucosinolates mehr... Arabidopsis Proteins 9064-51-1 Indoleacetic Acids Glucans
Beschreibung
Zusammenfassung:© 2025 The Author(s). New Phytologist © 2025 New Phytologist Foundation.
Plasmodesmata (PD) allow direct communication across the cellulosic plant cell wall, facilitating the intercellular movement of metabolites and signaling molecules within the symplast. In Arabidopsis thaliana embryos with reduced levels of the chloroplast RNA helicase ISE2, intercellular trafficking and the number of branched PD were increased. We therefore investigated the relationship between altered ISE2 expression and intercellular trafficking. Gene expression analyses in Arabidopsis tissues where ISE2 expression was increased or decreased identified genes associated with the metabolism of glucosinolates (GLSs) as highly affected. Concomitant with changes in the expression of GLS-related genes, plants with abnormal ISE2 expression contained altered GLS metabolic profiles compared with wild-type (WT) counterparts. Indeed, changes in the expression of GLS-associated genes led to altered intercellular trafficking in Arabidopsis leaves. Exogenous application of GLSs but not their breakdown products also resulted in altered intercellular trafficking. These changes in trafficking may be mediated by callose levels at PD as exogenous GLS treatment was sufficient to modulate plasmodesmal callose in WT plants. Furthermore, auxin metabolism was perturbed in plants with increased indole-type GLS levels. These findings suggest that GLSs, which are themselves transported between cells via PD, can act on PD to regulate plasmodesmal trafficking capacity
Beschreibung:Date Completed 10.04.2025
Date Revised 10.04.2025
published: Print-Electronic
Citation Status MEDLINE
ISSN:1469-8137
DOI:10.1111/nph.70032