|
|
|
|
| LEADER |
01000caa a22002652c 4500 |
| 001 |
NLM38542275X |
| 003 |
DE-627 |
| 005 |
20250509121325.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
250508s2025 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1016/j.plantsci.2025.112459
|2 doi
|
| 028 |
5 |
2 |
|a pubmed25n1374.xml
|
| 035 |
|
|
|a (DE-627)NLM38542275X
|
| 035 |
|
|
|a (NLM)40064352
|
| 035 |
|
|
|a (PII)S0168-9452(25)00077-9
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Frittelli, Arianna
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Improving the agronomic performance of high-amylose durum wheat
|
| 264 |
|
1 |
|c 2025
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 13.04.2025
|
| 500 |
|
|
|a Date Revised 13.04.2025
|
| 500 |
|
|
|a published: Print-Electronic
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a Copyright © 2025 The Authors. Published by Elsevier B.V. All rights reserved.
|
| 520 |
|
|
|a High-amylose wheat has garnered significant attention from the food industry for its potential to produce low-glycaemic food products. It is well-established that there is a direct correlation between the amylose content in flour and the amount of resistant starch (RS) in foods. Recently, some research initiatives have successfully produced high-amylose durum wheat by targeting key enzymes in the amylopectin biosynthesis pathway, though this has resulted in a reduction in seed weight. This study aimed to develop durum wheat genotypes with enhanced nutritional and agronomic traits by pyramiding mutations in the SSIIa genes and the GW2-A1 null allele. A cross between Svevo SSIIa- and Kronos GW2-A1- was performed, and marker-assisted selection (MAS) strategies were employed to identify ten sister lines (GW2-A1-/SSIIa-). Biochemical analyses revealed that the GW2-A1-/SSIIa- genotypes exhibited significantly higher amylose and resistant starch (5-10-fold) levels compared to Svevo and GW2-A1- controls. Phenotypic analyses highlighted that GW2-A1-/SSIIa- lines showed a 50 % increase in hundred-grain weight (HGW) and improved grain size parameters compared to Svevo SSIIa-, though these values remained lower than Svevo and Kronos GW2-A1-. Yield per plot increased by 67 % compared to Svevo SSIIa- but was 30-40 % lower than Svevo and Kronos GW2-A1-. Gene expression analysis revealed upregulation of key starch biosynthesis genes (Susy2, UGPase) in GW2-A1-/SSIIa- lines, suggesting compensatory mechanisms for reduced starch content. Downregulation of TPS7 indicated potential limitations in trehalose-6-phosphate biosynthesis, which may influence starch accumulation. This study demonstrates that combining SSIIa and GW2-A1 null mutations can mitigate yield losses associated with high-amylose genotypes while maintaining elevated levels of resistant starch and dietary fiber
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Durum Wheat
|
| 650 |
|
4 |
|a Fibre
|
| 650 |
|
4 |
|a Grain Width and Weight 2 (GW2)
|
| 650 |
|
4 |
|a High Amylose
|
| 650 |
|
4 |
|a Nutritional Quality
|
| 650 |
|
4 |
|a Resistant Starch
|
| 650 |
|
4 |
|a Yield
|
| 650 |
|
7 |
|a Amylose
|2 NLM
|
| 650 |
|
7 |
|a 9005-82-7
|2 NLM
|
| 650 |
|
7 |
|a Plant Proteins
|2 NLM
|
| 650 |
|
7 |
|a Starch
|2 NLM
|
| 650 |
|
7 |
|a 9005-25-8
|2 NLM
|
| 700 |
1 |
|
|a Botticella, Ermelinda
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Palombieri, Samuela
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Metelli, Giulio
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Masci, Stefania
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Silvestri, Marco
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Lafiandra, Domenico
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Sestili, Francesco
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Plant science : an international journal of experimental plant biology
|d 1985
|g 355(2025) vom: 14. Mai, Seite 112459
|w (DE-627)NLM098174193
|x 1873-2259
|7 nnas
|
| 773 |
1 |
8 |
|g volume:355
|g year:2025
|g day:14
|g month:05
|g pages:112459
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1016/j.plantsci.2025.112459
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 355
|j 2025
|b 14
|c 05
|h 112459
|