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241208s2024 xx |||||o 00| ||eng c |
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|a 10.1016/j.pnmrs.2024.05.004
|2 doi
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|a pubmed24n1627.xml
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|a (NLM)39645350
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|a eng
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|a Tugarinov, Vitali
|e verfasserin
|4 aut
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|a The utility of small nutation angle 1H pulses for NMR studies of methyl-containing side-chain dynamics in proteins
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|c 2024
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 07.12.2024
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|a Date Revised 09.12.2024
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Published by Elsevier B.V.
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|a We describe the utility of small nutation angle (acute; <90°) 1H radiofrequency pulses for efficient manipulation of magnetization in selectively [13CH3]-labeled methyl groups of otherwise deuterated proteins. Focusing primarily on NMR applications that target either fast (pico-to-nanosecond) motions of the methyl group three-fold rotation axis, or slow (micro-to-millisecond) processes associated with chemical exchange, we show that significant simplification of the 13CH3 spin-system and, as a consequence, of NMR pulse schemes, may be achieved in certain cases by the proper choice of the flip-angle of the 1H acute-angle pulse. In other instances, appropriate adjustment of acute-angle 1H pulses permits optimization of the sensitivity of NMR experiments. The results of acute-angle pulse based NMR experiments are validated by comparison with well-established NMR techniques for the characterization of fast dynamics of methyl-containing side-chains and chemical exchange processes
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|a Journal Article
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|a Review
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|a Methyl NMR
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|a Methyl-bearing side-chain dynamics in proteins
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|a Small nutation angle (1)H pulses
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|a Proteins
|2 NLM
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1 |
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|a Clore, G Marius
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Progress in nuclear magnetic resonance spectroscopy
|d 1998
|g 144-145(2024) vom: 07. Nov., Seite 40-62
|w (DE-627)NLM098212745
|x 1873-3301
|7 nnns
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| 773 |
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|g volume:144-145
|g year:2024
|g day:07
|g month:11
|g pages:40-62
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|u http://dx.doi.org/10.1016/j.pnmrs.2024.05.004
|3 Volltext
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|d 144-145
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