The utility of small nutation angle 1H pulses for NMR studies of methyl-containing side-chain dynamics in proteins
Published by Elsevier B.V.
| Veröffentlicht in: | Progress in nuclear magnetic resonance spectroscopy. - 1998. - 144-145(2024) vom: 07. Nov., Seite 40-62 |
|---|---|
| 1. Verfasser: | |
| Weitere Verfasser: | |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2024
|
| Zugriff auf das übergeordnete Werk: | Progress in nuclear magnetic resonance spectroscopy |
| Schlagworte: | Journal Article Review Methyl NMR Methyl-bearing side-chain dynamics in proteins Small nutation angle (1)H pulses Proteins |
| Zusammenfassung: | Published by Elsevier B.V. We describe the utility of small nutation angle (acute; <90°) 1H radiofrequency pulses for efficient manipulation of magnetization in selectively [13CH3]-labeled methyl groups of otherwise deuterated proteins. Focusing primarily on NMR applications that target either fast (pico-to-nanosecond) motions of the methyl group three-fold rotation axis, or slow (micro-to-millisecond) processes associated with chemical exchange, we show that significant simplification of the 13CH3 spin-system and, as a consequence, of NMR pulse schemes, may be achieved in certain cases by the proper choice of the flip-angle of the 1H acute-angle pulse. In other instances, appropriate adjustment of acute-angle 1H pulses permits optimization of the sensitivity of NMR experiments. The results of acute-angle pulse based NMR experiments are validated by comparison with well-established NMR techniques for the characterization of fast dynamics of methyl-containing side-chains and chemical exchange processes |
|---|---|
| Beschreibung: | Date Completed 07.12.2024 Date Revised 09.12.2024 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1873-3301 |
| DOI: | 10.1016/j.pnmrs.2024.05.004 |