A Single-Cell RNA Sequencing Guided Multienzymatic Hydrogel Design for Self-Regenerative Repair in Diabetic Mandibular Defects

A Single-Cell RNA Sequencing Guided Multienzymatic Hydrogel Design for Self-Regenerative Repair in Diabetic Mandibular Defects

© 2024 Wiley‐VCH GmbH.

Bibliographische Detailangaben
Veröffentlicht in:Advanced materials (Deerfield Beach, Fla.). - 1998. - 36(2024), 50 vom: 01. Dez., Seite e2410962
1. Verfasser: Lin, Peihua (VerfasserIn)
Weitere Verfasser: Qian, Zhouyang, Liu, Shanbiao, Ye, Xin, Xue, Pengpeng, Shao, Yangjie, Zhao, Jing, Guan, Yunan, Liu, Zhichao, Chen, Yuhua, Wang, Qiyue, Yi, Zhigao, Zhu, Mingjian, Yu, Mengfei, Ling, Daishun, Li, Fangyuan
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2024
Zugriff auf das übergeordnete Werk:Advanced materials (Deerfield Beach, Fla.)
Schlagworte:Journal Article Ru nanozyme diabetic mandibular defects multienzymatic hydrogel self‐regenerative repair single‐cell RNA sequencing Hydrogels Nitric Oxide 31C4KY9ESH Reactive Oxygen Species
LEADER 01000caa a22002652 4500
001 NLM379229420
003 DE-627
005 20241212232447.0
007 cr uuu---uuuuu
008 241022s2024 xx |||||o 00| ||eng c
024 7 |a 10.1002/adma.202410962  |2 doi 
028 5 2 |a pubmed24n1629.xml 
035 |a (DE-627)NLM379229420 
035 |a (NLM)39436107 
040 |a DE-627  |b ger  |c DE-627  |e rakwb 
041 |a eng 
100 1 |a Lin, Peihua  |e verfasserin  |4 aut 
245 1 2 |a A Single-Cell RNA Sequencing Guided Multienzymatic Hydrogel Design for Self-Regenerative Repair in Diabetic Mandibular Defects 
264 1 |c 2024 
336 |a Text  |b txt  |2 rdacontent 
337 |a ƒaComputermedien  |b c  |2 rdamedia 
338 |a ƒa Online-Ressource  |b cr  |2 rdacarrier 
500 |a Date Completed 12.12.2024 
500 |a Date Revised 12.12.2024 
500 |a published: Print-Electronic 
500 |a Citation Status MEDLINE 
520 |a © 2024 Wiley‐VCH GmbH. 
520 |a Conventional bone tissue engineering materials struggle to reinstate physiological bone remodeling in a diabetic context, primarily due to the compromised repolarization of proinflammatory macrophages to anti-inflammatory macrophages. Here, leveraging single-cell RNA sequencing (scRNA-seq) technology, the pivotal role of nitric oxide (NO) and reactive oxygen species (ROS) is unveiled in impeding macrophage repolarization during physiological bone remodeling amidst diabetes. Guided by scRNA-seq analysis, we engineer a multienzymatic bone tissue engineering hydrogel scaffold (MEBTHS) composed is engineered of methylpropenylated gelatin hydrogel integrated with ruthenium nanozymes, possessing both Ru0 and Ru4+ components. This design facilitates efficient NO elimination via Ru0 while simultaneously exhibiting ROS scavenging properties through Ru4+. Consequently, MEBTHS orchestrates macrophage reprogramming by neutralizing ROS and reversing NO-mediated mitochondrial metabolism, thereby rejuvenating bone marrow-derived mesenchymal stem cells and endothelial cells within diabetic mandibular defects, producing newly formed bone with quality comparable to that of normal bone. The scRNA-seq guided multienzymatic hydrogel design fosters the restoration of self-regenerative repair, marking a significant advancement in bone tissue engineering 
650 4 |a Journal Article 
650 4 |a Ru nanozyme 
650 4 |a diabetic mandibular defects 
650 4 |a multienzymatic hydrogel 
650 4 |a self‐regenerative repair 
650 4 |a single‐cell RNA sequencing 
650 7 |a Hydrogels  |2 NLM 
650 7 |a Nitric Oxide  |2 NLM 
650 7 |a 31C4KY9ESH  |2 NLM 
650 7 |a Reactive Oxygen Species  |2 NLM 
700 1 |a Qian, Zhouyang  |e verfasserin  |4 aut 
700 1 |a Liu, Shanbiao  |e verfasserin  |4 aut 
700 1 |a Ye, Xin  |e verfasserin  |4 aut 
700 1 |a Xue, Pengpeng  |e verfasserin  |4 aut 
700 1 |a Shao, Yangjie  |e verfasserin  |4 aut 
700 1 |a Zhao, Jing  |e verfasserin  |4 aut 
700 1 |a Guan, Yunan  |e verfasserin  |4 aut 
700 1 |a Liu, Zhichao  |e verfasserin  |4 aut 
700 1 |a Chen, Yuhua  |e verfasserin  |4 aut 
700 1 |a Wang, Qiyue  |e verfasserin  |4 aut 
700 1 |a Yi, Zhigao  |e verfasserin  |4 aut 
700 1 |a Zhu, Mingjian  |e verfasserin  |4 aut 
700 1 |a Yu, Mengfei  |e verfasserin  |4 aut 
700 1 |a Ling, Daishun  |e verfasserin  |4 aut 
700 1 |a Li, Fangyuan  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t Advanced materials (Deerfield Beach, Fla.)  |d 1998  |g 36(2024), 50 vom: 01. Dez., Seite e2410962  |w (DE-627)NLM098206397  |x 1521-4095  |7 nnns 
773 1 8 |g volume:36  |g year:2024  |g number:50  |g day:01  |g month:12  |g pages:e2410962 
856 4 0 |u http://dx.doi.org/10.1002/adma.202410962  |3 Volltext 
912 |a GBV_USEFLAG_A 
912 |a SYSFLAG_A 
912 |a GBV_NLM 
912 |a GBV_ILN_350 
951 |a AR 
952 |d 36  |j 2024  |e 50  |b 01  |c 12  |h e2410962