Warming triggers stomatal opening by enhancement of photosynthesis and ensuing guard cell CO2 sensing, whereas higher temperatures induce a photosynthesis-uncoupled response
© 2024 The Author(s). New Phytologist © 2024 New Phytologist Foundation.
| Veröffentlicht in: | The New phytologist. - 1979. - 244(2024), 5 vom: 06. Nov., Seite 1847-1863 |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2024
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| Zugriff auf das übergeordnete Werk: | The New phytologist |
| Schlagworte: | Journal Article blue light carbon dioxide guard cell heat heat stress photosynthesis protein kinase stomata Carbon Dioxide mehr... |
| Zusammenfassung: | © 2024 The Author(s). New Phytologist © 2024 New Phytologist Foundation. Plants integrate environmental stimuli to optimize photosynthesis vs water loss by controlling stomatal apertures. However, stomatal responses to temperature elevation and the underlying molecular genetic mechanisms remain less studied. We developed an approach for clamping leaf-to-air vapor pressure difference (VPDleaf) to fixed values, and recorded robust reversible warming-induced stomatal opening in intact plants. We analyzed stomatal temperature responses of mutants impaired in guard cell signaling pathways for blue light, abscisic acid (ABA), CO2, and the temperature-sensitive proteins, Phytochrome B (phyB) and EARLY-FLOWERING-3 (ELF3). We confirmed that phot1-5/phot2-1 leaves lacking blue-light photoreceptors showed partially reduced warming-induced stomatal opening. Furthermore, ABA-biosynthesis, phyB, and ELF3 were not essential for the stomatal warming response. Strikingly, Arabidopsis (dicot) and Brachypodium distachyon (monocot) mutants lacking guard cell CO2 sensors and signaling mechanisms, including ht1, mpk12/mpk4-gc, and cbc1/cbc2 abolished the stomatal warming response, suggesting a conserved mechanism across diverse plant lineages. Moreover, warming rapidly stimulated photosynthesis, resulting in a reduction in intercellular (CO2). Interestingly, further enhancing heat stress caused stomatal opening uncoupled from photosynthesis. We provide genetic and physiological evidence that the stomatal warming response is triggered by increased CO2 assimilation and stomatal CO2 sensing. Additionally, increasing heat stress functions via a distinct photosynthesis-uncoupled stomatal opening pathway |
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| Beschreibung: | Date Completed 07.11.2024 Date Revised 07.11.2024 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1469-8137 |
| DOI: | 10.1111/nph.20121 |