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241003s2024 xx |||||o 00| ||eng c |
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|a 10.1016/j.plaphy.2024.109147
|2 doi
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|a pubmed24n1598.xml
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|a (DE-627)NLM37842520X
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|a (NLM)39353294
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|a (PII)S0981-9428(24)00815-5
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Hou, Xiaoyang
|e verfasserin
|4 aut
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|a Transcription factor gene TaWRKY76 confers plants improved drought and salt tolerance through modulating stress defensive-associated processes in Triticum aestivum L
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|c 2024
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 07.11.2024
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|a Date Revised 07.11.2024
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2024. Published by Elsevier Masson SAS.
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|a WRKY transcription factor (TF) family acts as essential regulators in plant growth and abiotic stress responses. This study reported the function of TaWRKY76, a member of WRKY TF family in Triticum aestivum L., in regulating plant osmotic stress tolerance. TaWRKY76 transcripts were significantly upregulated upon drought and salt signaling, with dose extent- and stress temporal-dependent manners. Plant GUS activity assays suggested that stress responsive cis-acting elements, such as DRE and ABRE, exert essential roles in defining gene transcription under osmotic stress conditions. The TaWRKY76 protein targeted onto nucleus and possessed ability interacting with TaMYC2, a MYC TF member of wheat. TaWRKY76 and TaMYC2 positively regulated plant drought and salt adaptation by modulating osmotic stress-related physiological indices, including osmolyte contents, stomata movement, root morphology, and reactive oxygen species (ROS) homeostasis. Yeast one-hybrid assay indicated the binding ability of TaWRKY76 with promoters of TaDREB1;1, TaNCEB3, and TaCOR15;4. ChIP-PCR analysis confirmed that the osmotic stress genes are transcriptionally regulated by TaWRKY76. Moreover, the transgenic lines with knockdown of these stress-response genes displayed lowered plant biomass together with worsened root growth traits, decreased proline contents, and elevated ROS amounts. These results suggested that these stress defensive genes contributed to TaWRKY76-modulated osmotic stress tolerance. Highly positive correlations were observed between yield and the transcripts of TaWRKY76 in a wheat variety panel under field drought condition. A major haplotype TaWRKY76 Hap1 conferred improved drought tolerance. Our results suggested that TaWRKY76 is essential in plant drought and salt adaptation and a valuable target for molecular breeding stress-tolerant cultivars in Triticum aestivum L
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|a Journal Article
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|a Gene expression
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|a Osmotic stress
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|a Physiological indices
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|a Transgene analysis
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|a Wheat WRKY76
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|a Plant Proteins
|2 NLM
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| 650 |
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|a Transcription Factors
|2 NLM
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| 650 |
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|a Reactive Oxygen Species
|2 NLM
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| 700 |
1 |
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|a Ma, Chunying
|e verfasserin
|4 aut
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|a Wang, Ziyi
|e verfasserin
|4 aut
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|a Shi, Xinxin
|e verfasserin
|4 aut
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|a Duan, Wanrong
|e verfasserin
|4 aut
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| 700 |
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|a Fu, Xiaoxin
|e verfasserin
|4 aut
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| 700 |
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|a Liu, Jinzhi
|e verfasserin
|4 aut
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| 700 |
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|a Guo, Chengjin
|e verfasserin
|4 aut
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| 700 |
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|a Xiao, Kai
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 216(2024) vom: 22. Nov., Seite 109147
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnns
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| 773 |
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|g volume:216
|g year:2024
|g day:22
|g month:11
|g pages:109147
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|u http://dx.doi.org/10.1016/j.plaphy.2024.109147
|3 Volltext
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