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240929s2024 xx |||||o 00| ||eng c |
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|a 10.1021/acs.langmuir.4c02471
|2 doi
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|a pubmed24n1561.xml
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|a eng
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|a Tchounwou, Christine
|e verfasserin
|4 aut
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|a Mixtures of Intrinsically Disordered Neuronal Protein Tau and Anionic Liposomes Reveal Distinct Anionic Liposome-Tau Complexes Coexisting with Tau Liquid-Liquid Phase-Separated Coacervates
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|c 2024
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|a Text
|b txt
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|a Date Completed 08.10.2024
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|a Date Revised 08.10.2024
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|a published: Print-Electronic
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|a UpdateOf: bioRxiv. 2024 Jul 17:2024.07.15.603342. doi: 10.1101/2024.07.15.603342. - PMID 39071287
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|a Citation Status MEDLINE
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|a Tau, an intrinsically disordered neuronal protein and polyampholyte with an overall positive charge, is a microtubule (MT) associated protein that binds to anionic domains of MTs and suppresses their dynamic instability. Aberrant tau-MT interactions are implicated in Alzheimer's and other neurodegenerative diseases. Here, we studied the interactions between full-length human protein tau and other negatively charged binding substrates, as revealed by differential interference contrast (DIC) and fluorescence microscopy. As a binding substrate, we chose anionic liposomes (ALs) containing either 1,2-dioleoyl-sn-glycero-3-phosphatidylserine (DOPS, -1e) or 1,2-dioleoyl-sn-glycero-3-phosphatidylglycerol (DOPG, -1e) mixed with zwitterionic 1,2-dioleoyl-sn-glycero-3-phosphatidylcholine (DOPC) to mimic anionic plasma membranes of axons where tau resides. At low salt concentrations (0 to 10 mM KCl or NaCl) with minimal charge screening, reaction mixtures of tau and ALs resulted in the formation of distinct states of AL-tau complexes coexisting with liquid-liquid phase-separated tau self-coacervates arising from the polyampholytic nature of tau containing cationic and anionic domains. AL-tau complexes (i.e. tau-lipoplexes) exhibited distinct types of morphologies. This included large ∼20-30 μm tau-decorated giant vesicles with additional smaller liposomes with bound tau attached to the giant vesicles and tau-mediated finite-size assemblies of small liposomes. As the salt concentration was increased to near and above 150 mM for 1:1 electrolytes, AL-tau complexes remained stable, while tau self-coacervate droplets were found to dissolve, indicative of the breaking of (anionic/cationic) electrostatic bonds between tau chains due to increased charge screening. The findings are consistent with the hypothesis that distinct cationic domains of tau may interact with anionic lipid domains of the lumen-facing monolayer of the axon's plasma membrane, suggesting the possibility of transient yet robust interactions near relevant ionic strengths found in neurons
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|a Journal Article
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|a tau Proteins
|2 NLM
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|a Liposomes
|2 NLM
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|a Anions
|2 NLM
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|a Phosphatidylcholines
|2 NLM
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|a 1,2-dioleoylphosphatidylserine
|2 NLM
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|a 70614-14-1
|2 NLM
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|a Phosphatidylserines
|2 NLM
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|a 1,2-oleoylphosphatidylcholine
|2 NLM
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|a EDS2L3ODLV
|2 NLM
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| 650 |
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|a Phosphatidylglycerols
|2 NLM
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|a 1,2-dioleoyl-sn-glycero-3-phosphoglycerol
|2 NLM
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|a 66322-31-4
|2 NLM
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|a Intrinsically Disordered Proteins
|2 NLM
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|a Jobanputra, Anjali J
|e verfasserin
|4 aut
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|a Lasher, Dylan
|e verfasserin
|4 aut
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| 700 |
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|a Fletcher, Bretton J
|e verfasserin
|4 aut
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|a Jacinto, Jorge
|e verfasserin
|4 aut
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|a Bhaduri, Arjun
|e verfasserin
|4 aut
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|a Best, Rebecca L
|e verfasserin
|4 aut
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|a Fisher, William S
|e verfasserin
|4 aut
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|a Ewert, Kai K
|e verfasserin
|4 aut
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|a Li, Youli
|e verfasserin
|4 aut
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|a Feinstein, Stuart C
|e verfasserin
|4 aut
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| 700 |
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|a Safinya, Cyrus R
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1999
|g 40(2024), 40 vom: 08. Okt., Seite 21041-21051
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
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|g volume:40
|g year:2024
|g number:40
|g day:08
|g month:10
|g pages:21041-21051
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|u http://dx.doi.org/10.1021/acs.langmuir.4c02471
|3 Volltext
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