A standardized protocol for sample preparation for scanning electron microscopy to visualize extrachromosomal DNA
Extrachromosomal DNA (ecDNA) are circular DNA structures associated with cancer and drug resistance. One specific type, double minute (DM) chromosomes, has been studied since the 1960s using imaging techniques like cytogenetics and fluorescence microscopy. Specialized techniques such as atomic force...
| Veröffentlicht in: | BioTechniques. - 1988. - 76(2024), 7 vom: 26., Seite 311-321 |
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| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2024
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Journal Article correlative light and electron microscopy double minute chromosomes extrachromosomal DNA fluorescence microscopy scanning electron microscopy DNA, Circular DNA 9007-49-2 |
| Zusammenfassung: | Extrachromosomal DNA (ecDNA) are circular DNA structures associated with cancer and drug resistance. One specific type, double minute (DM) chromosomes, has been studied since the 1960s using imaging techniques like cytogenetics and fluorescence microscopy. Specialized techniques such as atomic force microscopy (AFM) and scanning electron microscopy (SEM) offer micro to nano-scale visualization, but current sample preparation methods may not optimally preserve ecDNA structure. Our study introduces a systematic protocol using SEM for high-resolution ecDNA visualization. We have optimized the end-to-end procedure, providing a standardized approach to explore the circular architecture of ecDNA and address the urgent need for better understanding in cancer research |
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| Beschreibung: | Date Completed 26.08.2024 Date Revised 26.08.2024 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1940-9818 |
| DOI: | 10.1080/07366205.2024.2346042 |