Sirt5-mediated lysine desuccinylation regulates oxidative stress adaptation in Magnaporthe oryzae during host intracellular infection

Sirt5-mediated lysine desuccinylation regulates oxidative stress adaptation in Magnaporthe oryzae during host intracellular infection

© 2024 The Authors. New Phytologist © 2024 New Phytologist Foundation.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 242(2024), 3 vom: 13. Apr., Seite 1257-1274
1. Verfasser: Ren, Zhiyong (VerfasserIn)
Weitere Verfasser: Dong, Xiang, Guan, Lun, Yang, Lei, Liu, Caiyun, Cai, Xuan, Hu, Hong, Lv, Ziwei, Liu, Hao, Zheng, Lu, Huang, Junbin, Wilson, Richard A, Chen, Xiao-Lin
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2024
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Magnaporthe oryzae ROS detoxification Sirt5 desuccinylation oxidative stress Reactive Oxygen Species Lysine K3Z4F929H6 Fungal Proteins
Beschreibung
Zusammenfassung:© 2024 The Authors. New Phytologist © 2024 New Phytologist Foundation.
Plant pathogenic fungi elaborate numerous detoxification strategies to suppress host reactive oxygen species (ROS), but their coordination is not well-understood. Here, we show that Sirt5-mediated protein desuccinylation in Magnaporthe oryzae is central to host ROS detoxification. SIRT5 encodes a desuccinylase important for virulence via adaptation to host oxidative stress. Quantitative proteomics analysis identified a large number of succinylated proteins targeted by Sirt5, most of which were mitochondrial proteins involved in oxidative phosphorylation, TCA cycle, and fatty acid oxidation. Deletion of SIRT5 resulted in hypersuccinylation of detoxification-related enzymes, and significant reduction in NADPH : NADP+ and GSH : GSSG ratios, disrupting redox balance and impeding invasive growth. Sirt5 desuccinylated thioredoxin Trx2 and glutathione peroxidase Hyr1 to activate their enzyme activity, likely by affecting proper folding. Altogether, this work demonstrates the importance of Sirt5-mediated desuccinylation in controlling fungal process required for detoxifying host ROS during M. oryzae infection
Beschreibung:Date Completed 12.04.2024
Date Revised 12.04.2024
published: Print-Electronic
Citation Status MEDLINE
ISSN:1469-8137
DOI:10.1111/nph.19683