Comparing the immune abnormalities in MIS-C to healthy children and those with inflammatory disease reveals distinct inflammatory cytokine production and a monofunctional T cell response

Bibliographische Detailangaben
Veröffentlicht in:	Clinical immunology (Orlando, Fla.). - 1999. - 259(2024) vom: 06. Feb., Seite 109877
1. Verfasser:	Butters, Claire (VerfasserIn)
Weitere Verfasser:	Benede, Ntombi, Moyo-Gwete, Thandeka, Richardson, Simone I, Rohlwink, Ursula, Shey, Muki, Ayres, Frances, Manamela, Nelia P, Makhado, Zanele, Balla, Sashkia R, Madzivhandila, Mashudu, Ngomti, Amkele, Baguma, Richard, Facey-Thomas, Heidi, Spracklen, Timothy F, Day, Jonathan, van der Ross, Hamza, Riou, Catherine, Burgers, Wendy A, Scott, Christiaan, Zühlke, Liesl, Moore, Penny L, Keeton, Roanne S, Webb, Kate
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2024
Zugriff auf das übergeordnete Werk:	Clinical immunology (Orlando, Fla.)
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't Antibody effector function Inflammatory cytokine profile MIS-C SARS-CoV-2-specific T cells Cytokines Immunoglobulin G Antibodies, Viral

Beschreibung
Zusammenfassung:	Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved. Multisystem inflammatory syndrome in children (MIS-C) is a severe, hyperinflammatory disease that occurs after exposure to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The underlying immune pathology of MIS-C is incompletely understood, with limited data comparing MIS-C to clinically similar paediatric febrile diseases at presentation. SARS-CoV-2-specific T cell responses have not been compared in these groups to assess whether there is a T cell profile unique to MIS-C. In this study, we measured inflammatory cytokine concentration and SARS-CoV-2-specific humoral immunity and T cell responses in children with fever and suspected MIS-C at presentation (n = 83) where MIS-C was ultimately confirmed (n = 58) or another diagnosis was made (n = 25) and healthy children (n = 91). Children with confirmed MIS-C exhibited distinctly elevated serum IL-10, IL-6, and CRP at presentation. No differences were detected in SARS-CoV-2 spike IgG serum concentration, neutralisation capacity, antibody dependant cellular phagocytosis, antibody dependant cellular cytotoxicity or SARS-CoV-2-specific T cell frequency between the groups. Healthy SARS-CoV-2 seropositive children had a higher proportion of polyfunctional SARS-CoV-2-specific CD4+ T cells compared to children with MIS-C and those with other inflammatory or infectious diagnoses, who both presented a largely monofunctional SARS-CoV-2-specific CD4+ T cell profile. Treatment with steroids and/or intravenous immunoglobulins resulted in rapid reduction of inflammatory cytokines but did not affect the SARS-CoV-2-specific IgG or CD4+ T cell responses in MIS-C. In these data, MIS-C had a unique cytokine profile but not a unique SARS-CoV-2 specific humoral or T cell cytokine response
Beschreibung:	Date Completed 29.01.2024 Date Revised 31.07.2024 published: Print-Electronic Citation Status MEDLINE
ISSN:	1521-7035
DOI:	10.1016/j.clim.2023.109877