PHB3 interacts with BRI1 and BAK1 to mediate brassinosteroid signal transduction in Arabidopsis and tomato

PHB3 interacts with BRI1 and BAK1 to mediate brassinosteroid signal transduction in Arabidopsis and tomato

© 2023 The Authors New Phytologist © 2023 New Phytologist Foundation.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 241(2024), 4 vom: 19. Jan., Seite 1510-1524
1. Verfasser: Li, Cheng (VerfasserIn)
Weitere Verfasser: Zhang, Shan, Li, Jingjuan, Huang, Shuhua, Zhao, Tong, Lv, Siqi, Liu, Jianwei, Wang, Shufen, Liu, Xiaohui, He, Shen, Zhang, Yanfeng, Xiao, Fangming, Wang, Fengde, Gao, Jianwei, Wang, Xiaofeng
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2024
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Arabidopsis BRASSINOSTEROID-INSENSITIVE 1 BRI1 ASSOCIATED RECEPTOR KINASE 1 PROHIBITIN 3 brassinosteroid phosphorylation tomato Protein Serine-Threonine Kinases EC 2.7.11.1 mehr... Brassinosteroids Protein Kinases EC 2.7.- Arabidopsis Proteins Prohibitins Mutant Proteins BAK1 protein, Arabidopsis EC 2.7.1.- BRI1 protein, Arabidopsis
Beschreibung
Zusammenfassung:© 2023 The Authors New Phytologist © 2023 New Phytologist Foundation.
Brassinosteroids (BRs) are plant hormones that are essential in plant growth and development. BRASSINOSTEROID-INSENSITIVE 1 (BRI1) and BRI1 ASSOCIATED RECEPTOR KINASE 1 (BAK1), which are located on the plasma membrane, function as co-receptors that accept and transmit BR signals. PROHIBITIN 3 (PHB3) was identified in both BRI1 and BAK1 complexes by affinity purification and LC-MS/MS analysis. Biochemical data showed that BRI1/BAK1 interacted with PHB3 in vitro and in vivo. BRI1/BAK1 phosphorylated PHB3 in vitro. When the Thr-80 amino acid in PHB3 was mutated to Ala, the mutant protein was not phosphorylated by BRI1 and the mutant protein interaction with BRI1 was abolished in the yeast two-hybrid assay. BAK1 did not phosphorylate the mutant protein PHB3T54A . The loss-of-function phb3 mutant showed a weaker BR signal than the wild-type. Genetic analyses revealed that PHB3 is a BRI1/BAK1 downstream substrate that participates in BR signalling. PHB3 has five homozygous in tomato, and we named the closest to AtPHB3 as SlPHB3.1. Biochemical data showed that SlBRI1/SlSERK3A/SlSERK3B interacted with SlPHB3.1 and SlPHB3.3. The CRISPR-Cas9 method generated slphb3.1 mutant led to a BR signal stunted relatively in tomatoes. PHB3 is a new component of the BR signal pathway in both Arabidopsis and tomato
Beschreibung:Date Completed 26.01.2024
Date Revised 26.01.2024
published: Print-Electronic
Citation Status MEDLINE
ISSN:1469-8137
DOI:10.1111/nph.19469