PHB3 interacts with BRI1 and BAK1 to mediate brassinosteroid signal transduction in Arabidopsis and tomato

PHB3 interacts with BRI1 and BAK1 to mediate brassinosteroid signal transduction in Arabidopsis and tomato

© 2023 The Authors New Phytologist © 2023 New Phytologist Foundation.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 241(2024), 4 vom: 19. Jan., Seite 1510-1524
1. Verfasser: Li, Cheng (VerfasserIn)
Weitere Verfasser: Zhang, Shan, Li, Jingjuan, Huang, Shuhua, Zhao, Tong, Lv, Siqi, Liu, Jianwei, Wang, Shufen, Liu, Xiaohui, He, Shen, Zhang, Yanfeng, Xiao, Fangming, Wang, Fengde, Gao, Jianwei, Wang, Xiaofeng
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2024
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Arabidopsis BRASSINOSTEROID-INSENSITIVE 1 BRI1 ASSOCIATED RECEPTOR KINASE 1 PROHIBITIN 3 brassinosteroid phosphorylation tomato Protein Serine-Threonine Kinases EC 2.7.11.1 mehr... Brassinosteroids Protein Kinases EC 2.7.- Arabidopsis Proteins Prohibitins Mutant Proteins BAK1 protein, Arabidopsis EC 2.7.1.- BRI1 protein, Arabidopsis
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500 |a Date Completed 26.01.2024 
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520 |a © 2023 The Authors New Phytologist © 2023 New Phytologist Foundation. 
520 |a Brassinosteroids (BRs) are plant hormones that are essential in plant growth and development. BRASSINOSTEROID-INSENSITIVE 1 (BRI1) and BRI1 ASSOCIATED RECEPTOR KINASE 1 (BAK1), which are located on the plasma membrane, function as co-receptors that accept and transmit BR signals. PROHIBITIN 3 (PHB3) was identified in both BRI1 and BAK1 complexes by affinity purification and LC-MS/MS analysis. Biochemical data showed that BRI1/BAK1 interacted with PHB3 in vitro and in vivo. BRI1/BAK1 phosphorylated PHB3 in vitro. When the Thr-80 amino acid in PHB3 was mutated to Ala, the mutant protein was not phosphorylated by BRI1 and the mutant protein interaction with BRI1 was abolished in the yeast two-hybrid assay. BAK1 did not phosphorylate the mutant protein PHB3T54A . The loss-of-function phb3 mutant showed a weaker BR signal than the wild-type. Genetic analyses revealed that PHB3 is a BRI1/BAK1 downstream substrate that participates in BR signalling. PHB3 has five homozygous in tomato, and we named the closest to AtPHB3 as SlPHB3.1. Biochemical data showed that SlBRI1/SlSERK3A/SlSERK3B interacted with SlPHB3.1 and SlPHB3.3. The CRISPR-Cas9 method generated slphb3.1 mutant led to a BR signal stunted relatively in tomatoes. PHB3 is a new component of the BR signal pathway in both Arabidopsis and tomato 
650 4 |a Journal Article 
650 4 |a Arabidopsis 
650 4 |a BRASSINOSTEROID-INSENSITIVE 1 
650 4 |a BRI1 ASSOCIATED RECEPTOR KINASE 1 
650 4 |a PROHIBITIN 3 
650 4 |a brassinosteroid 
650 4 |a phosphorylation 
650 4 |a tomato 
650 7 |a Protein Serine-Threonine Kinases  |2 NLM 
650 7 |a EC 2.7.11.1  |2 NLM 
650 7 |a Brassinosteroids  |2 NLM 
650 7 |a Protein Kinases  |2 NLM 
650 7 |a EC 2.7.-  |2 NLM 
650 7 |a Arabidopsis Proteins  |2 NLM 
650 7 |a Prohibitins  |2 NLM 
650 7 |a Mutant Proteins  |2 NLM 
650 7 |a BAK1 protein, Arabidopsis  |2 NLM 
650 7 |a EC 2.7.1.-  |2 NLM 
650 7 |a BRI1 protein, Arabidopsis  |2 NLM 
650 7 |a EC 2.7.11.1  |2 NLM 
700 1 |a Zhang, Shan  |e verfasserin  |4 aut 
700 1 |a Li, Jingjuan  |e verfasserin  |4 aut 
700 1 |a Huang, Shuhua  |e verfasserin  |4 aut 
700 1 |a Zhao, Tong  |e verfasserin  |4 aut 
700 1 |a Lv, Siqi  |e verfasserin  |4 aut 
700 1 |a Liu, Jianwei  |e verfasserin  |4 aut 
700 1 |a Wang, Shufen  |e verfasserin  |4 aut 
700 1 |a Liu, Xiaohui  |e verfasserin  |4 aut 
700 1 |a He, Shen  |e verfasserin  |4 aut 
700 1 |a Zhang, Yanfeng  |e verfasserin  |4 aut 
700 1 |a Xiao, Fangming  |e verfasserin  |4 aut 
700 1 |a Wang, Fengde  |e verfasserin  |4 aut 
700 1 |a Gao, Jianwei  |e verfasserin  |4 aut 
700 1 |a Wang, Xiaofeng  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t The New phytologist  |d 1979  |g 241(2024), 4 vom: 19. Jan., Seite 1510-1524  |w (DE-627)NLM09818248X  |x 1469-8137  |7 nnns 
773 1 8 |g volume:241  |g year:2024  |g number:4  |g day:19  |g month:01  |g pages:1510-1524 
856 4 0 |u http://dx.doi.org/10.1111/nph.19469  |3 Volltext 
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