Complete pathway elucidation and heterologous reconstitution of (+)-nootkatone biosynthesis from Alpinia oxyphylla

Complete pathway elucidation and heterologous reconstitution of (+)-nootkatone biosynthesis from Alpinia oxyphylla

© 2023 The Authors New Phytologist © 2023 New Phytologist Foundation.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 241(2024), 2 vom: 11. Jan., Seite 779-792
1. Verfasser: Deng, Xiaomin (VerfasserIn)
Weitere Verfasser: Ye, Ziling, Duan, Jingyu, Chen, Fangfang, Zhi, Yao, Huang, Man, Huang, Minjian, Cheng, Weijia, Dou, Yujie, Kuang, Zhaolin, Huang, Yanglei, Bian, Guangkai, Deng, Zixin, Liu, Tiangang, Lu, Li
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2024
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article (+)-nootkatone (+)-valencene Alpinia oxyphylla Saccharomyces cerevisiae plant specialised metabolic pathway sesquiterpenoid biosynthesis valencene 96H21P91IG nootkatone mehr... IZ2Y119N4J Sesquiterpenes
LEADER 01000caa a22002652c 4500
001 NLM364252340
003 DE-627
005 20250305102440.0
007 cr uuu---uuuuu
008 231226s2024 xx |||||o 00| ||eng c
024 7 |a 10.1111/nph.19375  |2 doi 
028 5 2 |a pubmed25n1213.xml 
035 |a (DE-627)NLM364252340 
035 |a (NLM)37933426 
040 |a DE-627  |b ger  |c DE-627  |e rakwb 
041 |a eng 
100 1 |a Deng, Xiaomin  |e verfasserin  |4 aut 
245 1 0 |a Complete pathway elucidation and heterologous reconstitution of (+)-nootkatone biosynthesis from Alpinia oxyphylla 
264 1 |c 2024 
336 |a Text  |b txt  |2 rdacontent 
337 |a ƒaComputermedien  |b c  |2 rdamedia 
338 |a ƒa Online-Ressource  |b cr  |2 rdacarrier 
500 |a Date Completed 22.12.2023 
500 |a Date Revised 22.12.2023 
500 |a published: Print-Electronic 
500 |a RefSeq: NP_001239041, AY561843, AAQ04608, AFN21429, AYH59748.1, BAT70338, AGJ03150, NP_001312854, E1B2Z9, A6YIH8 
500 |a Citation Status MEDLINE 
520 |a © 2023 The Authors New Phytologist © 2023 New Phytologist Foundation. 
520 |a (+)-Nootkatone is a natural sesquiterpene ketone widely used in food, cosmetics, pharmaceuticals, and agriculture. It is also regarded as one of the most valuable terpenes used commercially. However, plants contain trace amounts of (+)-nootkatone, and extraction from plants is insufficient to meet market demand. Alpinia oxyphylla is a well-known medicinal plant in China, and (+)-nootkatone is one of the main components within the fruits. By transcriptome mining and functional screening using a precursor-providing yeast chassis, the complete (+)-nootkatone biosynthetic pathway in Alpinia oxyphylla was identified. A (+)-valencene synthase (AoVS) was identified as a novel monocot-derived valencene synthase; three (+)-valencene oxidases AoCYP6 (CYP71BB2), AoCYP9 (CYP71CX8), and AoCYP18 (CYP701A170) were identified by constructing a valencene-providing yeast strain. With further characterisation of a cytochrome P450 reductase (AoCPR1) and three dehydrogenases (AoSDR1/2/3), we successfully reconstructed the (+)-nootkatone biosynthetic pathway in Saccharomyces cerevisiae, representing a basis for its biotechnological production. Identifying the biosynthetic pathway of (+)-nootkatone in A. oxyphylla unravelled the molecular mechanism underlying its formation in planta and also supported the bioengineering production of (+)-nootkatone. The highly efficient yeast chassis screening method could be used to elucidate the complete biosynthetic pathway of other valuable plant natural products in future 
650 4 |a Journal Article 
650 4 |a (+)-nootkatone 
650 4 |a (+)-valencene 
650 4 |a Alpinia oxyphylla 
650 4 |a Saccharomyces cerevisiae 
650 4 |a plant specialised metabolic pathway 
650 4 |a sesquiterpenoid biosynthesis 
650 7 |a valencene  |2 NLM 
650 7 |a 96H21P91IG  |2 NLM 
650 7 |a nootkatone  |2 NLM 
650 7 |a IZ2Y119N4J  |2 NLM 
650 7 |a Sesquiterpenes  |2 NLM 
700 1 |a Ye, Ziling  |e verfasserin  |4 aut 
700 1 |a Duan, Jingyu  |e verfasserin  |4 aut 
700 1 |a Chen, Fangfang  |e verfasserin  |4 aut 
700 1 |a Zhi, Yao  |e verfasserin  |4 aut 
700 1 |a Huang, Man  |e verfasserin  |4 aut 
700 1 |a Huang, Minjian  |e verfasserin  |4 aut 
700 1 |a Cheng, Weijia  |e verfasserin  |4 aut 
700 1 |a Dou, Yujie  |e verfasserin  |4 aut 
700 1 |a Kuang, Zhaolin  |e verfasserin  |4 aut 
700 1 |a Huang, Yanglei  |e verfasserin  |4 aut 
700 1 |a Bian, Guangkai  |e verfasserin  |4 aut 
700 1 |a Deng, Zixin  |e verfasserin  |4 aut 
700 1 |a Liu, Tiangang  |e verfasserin  |4 aut 
700 1 |a Lu, Li  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t The New phytologist  |d 1979  |g 241(2024), 2 vom: 11. Jan., Seite 779-792  |w (DE-627)NLM09818248X  |x 1469-8137  |7 nnas 
773 1 8 |g volume:241  |g year:2024  |g number:2  |g day:11  |g month:01  |g pages:779-792 
856 4 0 |u http://dx.doi.org/10.1111/nph.19375  |3 Volltext 
912 |a GBV_USEFLAG_A 
912 |a SYSFLAG_A 
912 |a GBV_NLM 
912 |a GBV_ILN_350 
951 |a AR 
952 |d 241  |j 2024  |e 2  |b 11  |c 01  |h 779-792