Complete pathway elucidation and heterologous reconstitution of (+)-nootkatone biosynthesis from Alpinia oxyphylla
© 2023 The Authors New Phytologist © 2023 New Phytologist Foundation.
| Publié dans: | The New phytologist. - 1979. - 241(2024), 2 vom: 11. Jan., Seite 779-792 |
|---|---|
| Auteur principal: | |
| Autres auteurs: | , , , , , , , , , , , , , |
| Format: | Article en ligne |
| Langue: | English |
| Publié: |
2024
|
| Accès à la collection: | The New phytologist |
| Sujets: | Journal Article (+)-nootkatone (+)-valencene Alpinia oxyphylla Saccharomyces cerevisiae plant specialised metabolic pathway sesquiterpenoid biosynthesis valencene 96H21P91IG nootkatone plus... |
| Résumé: | © 2023 The Authors New Phytologist © 2023 New Phytologist Foundation. (+)-Nootkatone is a natural sesquiterpene ketone widely used in food, cosmetics, pharmaceuticals, and agriculture. It is also regarded as one of the most valuable terpenes used commercially. However, plants contain trace amounts of (+)-nootkatone, and extraction from plants is insufficient to meet market demand. Alpinia oxyphylla is a well-known medicinal plant in China, and (+)-nootkatone is one of the main components within the fruits. By transcriptome mining and functional screening using a precursor-providing yeast chassis, the complete (+)-nootkatone biosynthetic pathway in Alpinia oxyphylla was identified. A (+)-valencene synthase (AoVS) was identified as a novel monocot-derived valencene synthase; three (+)-valencene oxidases AoCYP6 (CYP71BB2), AoCYP9 (CYP71CX8), and AoCYP18 (CYP701A170) were identified by constructing a valencene-providing yeast strain. With further characterisation of a cytochrome P450 reductase (AoCPR1) and three dehydrogenases (AoSDR1/2/3), we successfully reconstructed the (+)-nootkatone biosynthetic pathway in Saccharomyces cerevisiae, representing a basis for its biotechnological production. Identifying the biosynthetic pathway of (+)-nootkatone in A. oxyphylla unravelled the molecular mechanism underlying its formation in planta and also supported the bioengineering production of (+)-nootkatone. The highly efficient yeast chassis screening method could be used to elucidate the complete biosynthetic pathway of other valuable plant natural products in future |
|---|---|
| Description: | Date Completed 22.12.2023 Date Revised 22.12.2023 published: Print-Electronic RefSeq: NP_001239041, AY561843, AAQ04608, AFN21429, AYH59748.1, BAT70338, AGJ03150, NP_001312854, E1B2Z9, A6YIH8 Citation Status MEDLINE |
| ISSN: | 1469-8137 |
| DOI: | 10.1111/nph.19375 |