Investigating seed bank potential of crustose coralline algae using DNA metabarcoding
© 2023 The Authors. Journal of Phycology published by Wiley Periodicals LLC on behalf of Phycological Society of America.
| Publié dans: | Journal of phycology. - 1966. - 60(2024), 1 vom: 21. Feb., Seite 195-202 |
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| Auteur principal: | |
| Autres auteurs: | , |
| Format: | Article en ligne |
| Langue: | English |
| Publié: |
2024
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| Accès à la collection: | Journal of phycology |
| Sujets: | Journal Article Research Support, Non-U.S. Gov't DNA metabarcoding algae crustose coralline algae diversity seed bank RNA, Ribosomal, 16S RNA, Ribosomal, 23S |
| Résumé: | © 2023 The Authors. Journal of Phycology published by Wiley Periodicals LLC on behalf of Phycological Society of America. To examine the potential for the autogenic ecosystem engineers, crustose coralline algae (CCA), to serve as seed banks or refugia for life stages of other species, it is critical to develop sampling protocols that reflect the diversity of life present. In this pilot study on two shallow water species of CCA collected from Raoul Island (Kermadec Islands; Rangitāhua) New Zealand, we investigated two preservation methods (ethanol vs. silica gel), sampled inner and outer regions of the crusts, and used DNA metabarcoding and seven genes/gene regions (16S rRNA, 18S rRNA, 23S rRNA, cox1, rbcL, and tufA genes and the ITS rRNA region) to develop a protocol for taxa identification. The results revealed immense diversity, with typically more taxa identified within the inner layers than the outer layers. As highlighted in other metabarcoding studies and in earlier work on rhodoliths (nodose coralline algae), reference databases are incomplete, and to some extent, the use of multiple markers mitigates this issue. Specifically, the 23S rRNA and rbcL genes are currently more suitable for identifying algae, while the cox1 gene fares better at capturing the diversity present inclusive of algae. Further investigation of these autogenic ecosystem engineers that likely act as marine seed banks is needed |
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| Description: | Date Completed 19.02.2024 Date Revised 15.04.2024 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1529-8817 |
| DOI: | 10.1111/jpy.13403 |