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231226s2023 xx |||||o 00| ||eng c |
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|a 10.1016/j.plaphy.2023.108000
|2 doi
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|a pubmed24n1205.xml
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|a (NLM)37683585
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|a (PII)S0981-9428(23)00511-9
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|a DE-627
|b ger
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|e rakwb
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|a eng
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|a Li, Guo
|e verfasserin
|4 aut
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|a New insights into the genetic manipulation of the R2R3-MYB and CHI gene families on anthocyanin pigmentation in Petunia hybrida
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|c 2023
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Revised 21.10.2023
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|a published: Print-Electronic
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|a Citation Status Publisher
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|a Copyright © 2023 Elsevier Masson SAS. All rights reserved.
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|a Several R2R3-MYB genes control anthocyanin pigmentation in petunia, and ANTHOCYANIN-2 (AN2) is treated as the main player in petal limbs. However, the actual roles of R2R3-MYBs in the coloration of different floral tissues in the so called "darkly-veined" petunias are still not clear. The genetic background and expression of AN2 paralogs from various petunias with different color patterns were identified. All "darkly-veined" genotypes have the identical mutation in the AN2 gene, but express a different functional paralog - ANTHOCYANIN-4 (AN4) - abundantly in flowers. Constitutive overexpression of PhAN4 in this petunia resulted not only in a fully colored flower but also in a clearly visible pigmentation in the green tissue and roots, which can be rapidly increased by stress conditions. Suppression of AN4 gene resulted in discolored petals and whitish anthers. Interestingly, when a similar white flower phenotype was achieved by knockout of an essential structural gene of anthocyanin biosynthesis - CHALCONE ISOMERASE-A (CHI-A) - the plant responded directly by upregulating of another paralogs - DEEP PURPLE (DPL) and PURPLE HAZE (PHZ). Moreover, we also found that CHI-B can partially substitute for CHI-A in anthers, but not in vegetative tissues. Further, no significant effects on the longevity of white or enhanced colored flowers were observed compared with the wild type. We concluded that endogenous up-regulation of AN4 leads to the restoration of petal color in the "darkly-veined" phenotypes as a result of the breeding process under human selection, and CHI-B is a backup for CHI-A acitvity in some floral tissues
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|a Journal Article
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|a ANTHOCYANIN-4 (AN4)
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| 650 |
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|a CHALCONE ISOMERASE (CHI)
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| 650 |
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|a CRISPR/Cas9
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| 650 |
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|a Flower coloration
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| 650 |
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|a R2R3-MYB transcription factor
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| 650 |
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|a Sucrose induction
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| 700 |
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|a Michaelis, Dietz Felix
|e verfasserin
|4 aut
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| 700 |
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|a Huang, Junjie
|e verfasserin
|4 aut
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| 700 |
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|a Serek, Margrethe
|e verfasserin
|4 aut
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| 700 |
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|a Gehl, Christian
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 203(2023) vom: 01. Okt., Seite 108000
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnns
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| 773 |
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|g volume:203
|g year:2023
|g day:01
|g month:10
|g pages:108000
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|u http://dx.doi.org/10.1016/j.plaphy.2023.108000
|3 Volltext
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