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231226s2024 xx |||||o 00| ||eng c |
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|a 10.1002/adma.202303321
|2 doi
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|a pubmed24n1422.xml
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|a (DE-627)NLM360401953
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|a (NLM)37540501
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Yuan, Xiushuang
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Natural Wood-Derived Macroporous Cellulose for Highly Efficient and Ultrafast Elimination of Double-Stranded RNA from In Vitro-Transcribed mRNA
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|c 2024
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 29.05.2024
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|a Date Revised 29.05.2024
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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| 520 |
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|a © 2023 Wiley‐VCH GmbH.
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|a Double-stranded RNA (dsRNA) is a major impurity that can induce innate immune responses and cause adverse drug reactions. Removing dsRNA is an essential and non-trivial process in manufacturing mRNA. Current methods for dsRNA elimination use either high-performance liquid chromatography or microcrystalline cellulose, rendering the process complex, expensive, toxic, and/or time-consuming. This study introduces a highly efficient and ultrafast method for dsRNA elimination using natural wood-derived macroporous cellulose (WMC). With a naturally formed large total pore area and low tortuosity, WMC removes up to 98% dsRNA within 5 min. This significantly shortens the time for mRNA purification and improves purification efficiency. WMC can also be filled into chromatographic columns of different sizes and integrates with fast-protein liquid chromatography for large-scale mRNA purification to meet the requirements of mRNA manufacture. This study further shows that WMC purification improves the enhanced green fluorescent protein mRNA expression efficiency by over 28% and significantly reduces cytokine secretion and innate immune responses in the cells. Successfully applying WMC provides an ultrafast and efficient platform for mRNA purification, enabling large-scale production with significant cost reduction
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|a Journal Article
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|a 5‐min purification
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|a RNA purification
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|a eco‐friendly process
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|a macropores
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|a natural wood
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|a Cellulose
|2 NLM
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|a 9004-34-6
|2 NLM
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| 650 |
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|a RNA, Messenger
|2 NLM
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| 650 |
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|a RNA, Double-Stranded
|2 NLM
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| 650 |
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|a Green Fluorescent Proteins
|2 NLM
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| 650 |
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|a 147336-22-9
|2 NLM
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| 700 |
1 |
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|a Wu, Zhanfeng
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Guo, Jing
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Luo, Dengwang
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Li, Tianyao
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Cao, Qinghao
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Ren, Xiangyu
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Fang, Han
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Xu, Dawei
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Cao, Yuhong
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Advanced materials (Deerfield Beach, Fla.)
|d 1998
|g 36(2024), 22 vom: 02. Mai, Seite e2303321
|w (DE-627)NLM098206397
|x 1521-4095
|7 nnns
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| 773 |
1 |
8 |
|g volume:36
|g year:2024
|g number:22
|g day:02
|g month:05
|g pages:e2303321
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| 856 |
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|u http://dx.doi.org/10.1002/adma.202303321
|3 Volltext
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|d 36
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|e 22
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|h e2303321
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