Advancing In Situ Food Monitoring through a Smart Lab-in-a-Package System Demonstrated by the Detection of Salmonella in Whole Chicken

© 2023 The Authors. Advanced Materials published by Wiley-VCH GmbH.

Bibliographische Detailangaben
Veröffentlicht in:Advanced materials (Deerfield Beach, Fla.). - 1998. - 35(2023), 40 vom: 26. Okt., Seite e2302641
1. Verfasser: Prasad, Akansha (VerfasserIn)
Weitere Verfasser: Khan, Shadman, Monteiro, Jonathan K, Li, Jiuxing, Arshad, Fatima, Ladouceur, Liane, Tian, Lei, Shakeri, Amid, Filipe, Carlos D M, Li, Yingfu, Didar, Tohid F
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2023
Zugriff auf das übergeordnete Werk:Advanced materials (Deerfield Beach, Fla.)
Schlagworte:Journal Article Salmonella detection food contamination food sensing functional DNA probes in situ monitoring pathogen detection smart packaging
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520 |a With food production shifting away from traditional farm-to-table approaches to efficient multistep supply chains, the incidence of food contamination has increased. Consequently, pathogen testing via inefficient culture-based methods has increased, despite its lack of real-time capabilities and need for centralized facilities. While in situ pathogen detection would address these limitations and enable individual product monitoring, accurate detection within unprocessed, packaged food products without user manipulation has proven elusive. Herein, "Lab-in-a-Package" is presented, a platform capable of sampling, concentrating, and detecting target pathogens within closed food packaging, without intervention. This system consists of a newly designed packaging tray and reagent-infused membrane that can be paired universally with diverse pathogen sensors. The inclined food packaging tray maximizes fluid localization onto the sensing interface, while the membrane acts as a reagent-immobilizing matrix and an antifouling barrier for the sensor. The platform is substantiated using a newly discovered Salmonella-responsive nucleic acid probe, which enables hands-free detection of 103 colony forming units (CFU) g-1 target pathogen in a packaged whole chicken. The platform remains effective when contamination is introduced with toolsand surfaces, ensuring widespread efficacy. Its real-world use for in situ detection is simulated using a handheld fluorescence scanner with smartphone connectivity 
650 4 |a Journal Article 
650 4 |a Salmonella detection 
650 4 |a food contamination 
650 4 |a food sensing 
650 4 |a functional DNA probes 
650 4 |a in situ monitoring 
650 4 |a pathogen detection 
650 4 |a smart packaging 
700 1 |a Khan, Shadman  |e verfasserin  |4 aut 
700 1 |a Monteiro, Jonathan K  |e verfasserin  |4 aut 
700 1 |a Li, Jiuxing  |e verfasserin  |4 aut 
700 1 |a Arshad, Fatima  |e verfasserin  |4 aut 
700 1 |a Ladouceur, Liane  |e verfasserin  |4 aut 
700 1 |a Tian, Lei  |e verfasserin  |4 aut 
700 1 |a Shakeri, Amid  |e verfasserin  |4 aut 
700 1 |a Filipe, Carlos D M  |e verfasserin  |4 aut 
700 1 |a Li, Yingfu  |e verfasserin  |4 aut 
700 1 |a Didar, Tohid F  |e verfasserin  |4 aut 
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